LOCUS       sequence-103194-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and 
            Mxi1 domain controlled by TDH3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pTDH3-dCas9-Mxi1
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        24..690
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     560..583
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     regulatory      698..707
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             713..733
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gtctactagt cagttcgagt ttatcattat caatactgcc atttcaaaga atacgtaaat
       61 aattaatagt agtgattttc ctaactttat ttagtcaaaa aattagcctt ttaattctgc
      121 tgtaacccgt acatgcccaa aatagggggc gggttacaca gaatatataa catcgtaggt
      181 gtctgggtga acagtttatt cctggcatcc actaaatata atggagcccg ctttttaagc
      241 tggcatccag aaaaaaaaag aatcccagca ccaaaatatt gttttcttca ccaaccatca
      301 gttcataggt ccattctctt agcgcaacta cagagaacag gggcacaaac aggcaaaaaa
      361 cgggcacaac ctcaatggag tgatgcaacc tgcctggagt aaatgatgac acaaggcaat
      421 tgacccacgc atgtatctat ctcattttct tacaccttct attaccttct gctctctctg
      481 atttggaaaa agctgaaaaa aaaggttgaa accagttccc tgaaattatt cccctacttg
      541 actaataagt atataaagac ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa
      601 cttcttaaat tctactttta tagttagtct tttttttagt tttaaaacac caagaactta
      661 gtttcgaata aacacacata aacaaacaaa acccgccgcc accatggacg tcccaaagaa
      721 gaagagaaag gtaggcagcg acaagaagta ttctatcgga ctggccatcg ggactaatag
      781 cgtcgggtgg gccgtgatca ctgacgagta caaggtgccc tctaagaagt tcaaggtgct
      841 cgggaacacc gaccggcatt ccatc
//
