LOCUS       sequence-103196-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 plasmid with sgRNA deleted; for use in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Ward JD
  TITLE     Rapid and Precise Engineering of the Caenorhabditis elegans Genome 
            with Lethal Mutation Co-conversion and Inactivation of NHEJ Repair.
  JOURNAL   Genetics. 2014 Dec 9. pii: genetics.114.172361.
  PUBMED    25491644
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 Dec 9. pii: genetics.114.172361."
COMMENT     Sequence Label: pJW1259
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..30
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(68..90)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     97..114
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     98..114
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    122..142
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      complement(146..469)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(484..510)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(511..531)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
       61 gtgaaaacct ctgacacatg cagctcccgg cgacgttgta aaacgacggc cagtgaatta
      121 tcaactatgt ataataaagt tgtgagactt ttttcttggc ggcacaataa agtttcctct
      181 ttttgttggg tcactcaacg aacacaatac aaatatttga gcgctcgcat ttttctcaac
      241 ctttcgggtt taaagcactt caaaatggaa ttgaagagag attgtgaaag tacatcagac
      301 agaatcacat tttgatagca ttcacttcac tcagatgcaa acttttcagt gactataaaa
      361 agtcataaaa taataacatt aaaaaagcaa ataaattagc gagagaattt tttgacaaaa
      421 agaaagaaga gtgatagaga agaagggaat gcttgaaagg attttgcatt tatcactagt
      481 ttaggcgtag tctgggacgt cgtatgggta gaccttacgc ttcttctttg gtggtccgga
      541 tcctccgtct cctccgagtt gggagaggtc gatacgggtc tcgtagagtc cggtgatgga
      601 ttggtggatg agggtggcgt cgaggacctc cttggtggag gtgtaacgct tacggtcgat
      661 ggtggtgtcg aagtacttga aggcggctgg ggctccgagg ttggtgaggg tgaagaggtg
      721 gatgatgttc tcggcttgct cacggattgg cttgtcacgg tgcttgttgt aggcggagag
      781 gaccttgtcg aggttggcgt cggcgagg
//
