LOCUS       sequence-103206-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Dog p53 (residues 1-77) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Daughdrill GW, Borcherds WM, Wu H
  TITLE     Disorder predictors also predict backbone dynamics for a family of 
            disordered proteins.
  JOURNAL   PLoS One. 2011;6(12):e29207. doi: 10.1371/journal.pone.0029207. Epub
            2011 Dec 15.
  PUBMED    22195023
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e29207. doi"; pages: " 
            10.1371/journal.pone.0029207. Epub 2011 Dec 15"
COMMENT     Sequence Label: pET28-p53 (Dog 1-77)
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(279..296)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(306..323)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             342..364
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    379..403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(403..422)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(404..422)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(506..525)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtt aacttggggc ggggcctggg
       61 gcggtagggg cggaggtggc tggcatacgt ggggcatcgt cggagtcttc atctaaccaa
      121 tttacaacag attcggggag taacagttca tcaacagcag ggcagagttc ggaggagagc
      181 acattatttt cgggtaagag gttccataat tcactaaagg tttcttggga taaggggggg
      241 tcaatattta attctgattg agattcttcc atcatatggc tgccgcgcgg caccaggccg
      301 ctgctgtgat gatgatgatg atggctgctg cccatggtat atctccttct taaagttaaa
      361 caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat
      421 taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc
      481 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
      541 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
      601 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
      661 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
      721 cgagatcccg gacaccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc
      781 ggaagagagt caattcaggg
//
