LOCUS       sequence-103219-       13427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector for PITT landing pad knock-in into the Rosa26 
            locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13427)
  AUTHORS   Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, 
            Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
  TITLE     One-step generation of multiple transgenic mouse lines using an 
            improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
  JOURNAL   BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
  PUBMED    25887549
REFERENCE   2  (bases 1 to 13427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-04-9"; volume: "16:274. doi"; pages: " 
            10.1186/s12864-015-1432-5"
COMMENT     Sequence Label: pBDU
FEATURES             Location/Qualifiers
     source          1..13427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..643
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        625..643
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     intron          1240..1305
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1382..1954)
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     misc_feature    join(4184..4249,4250..4287)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     regulatory      4451..4460
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4457..4459,4460..4462,4463..5173)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4457..4459,4460..4462,4463..5173)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4502..4523)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4763..4782)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5110..5131
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5183..5236
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5237..6028
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5288..5307)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5898..5917
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6190..6271
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6227..6246)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(6272..6319)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    complement(6320..6353)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     misc_feature    6741..7390
                     /label=HA-R
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(11237..11256)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11238..11256)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11278..11294)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11278..11294)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11291..11313)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11302..11318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11326..11332,11333..11350,11351..11356))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11371..11392
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11509..11526)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11680..12268)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11760..11779)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12439..13230,13231..13299))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13062..13081
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13300..13404)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
      661 atggcaattg ggccgagcaa gcttgcatgc ggccgcatct agctagagtc gatcgaccag
      721 cttctgatgg aattagaact tggcaaaaca atactgagaa tgaagtgtat gtggaacaga
      781 ggctgctgat ctcgttcttc aggctatgaa actgacacat ttggaaacca cagtacttag
      841 aaccacaaag tgggaatcaa gagaaaaaca atgatcccac gagagatcta tagatctata
      901 gatcatgagt gggaggaatg agctggccct taatttggtt ttgcttgttt aaattatgat
      961 atccaactat gaaacattat cataaagcaa tagtaaagag ccttcagtaa agagcaggca
     1021 tttatctaat cccaccccac ccccaccccc gtagctccaa tccttccatt caaaatgtag
     1081 gtactctgtt ctcacccttc ttaacaaagt atgacaggaa aaacttccat tttagtggac
     1141 atctttattg tttaatagat catcaatttc tgcatccctc gactctagtg gatctgcatt
     1201 ccaccactgc tcccattcat cagttccata ggttggaatc taaaatacac aaacaattag
     1261 aatcagtagt ttaacacatt atacacttaa aaattttata tttaccttag agctttaaat
     1321 ctctgtaggt agtttgtcca attatgtcac accacagaag taaggttcct tcacaaagag
     1381 atcgcctgac acgatttcct gcacaggctt gagccatata ctcatacatc gcatcttggc
     1441 cacgttttcc acgggtttca aaattaatct caagttctac gcttaacgct ttcgcctgtt
     1501 cccagttatt aatatattca acgctagaac tcccctcagc gaagggaagg ctgagcacta
     1561 cacgcgaagc accatcaccg aaccttttga taaactcttc cgttccgact tgctccatca
     1621 acggttcagt gagacttaaa cctaactctt tcttaatagt ttcggcatta tccactttta
     1681 gtgcgagaac cttcgtcagt cctggatacg tcactttgac cacgcctcca gcttttccag
     1741 agagcgggtt ttcattatct acagagtatc ccgcagcgtc gtatttattg tcggtactat
     1801 aaaacccttt ccaatcatcg tcataatttc cttgtgtacc agattttggc ttttgtatac
     1861 ctttttgaat ggaatctaca taaccaggtt tagtcccgtg gtacgaagaa aagttttcca
     1921 tcacaaaaga tttagaagaa tcaacaacat catcaggatc catggcgagg acctgcaggg
     1981 tcgctcggtg ttcgaggcca cacgcgtcac cttaatatgc gaagtggacc tgggaccgcg
     2041 ccgccccgac tgcatctgcg tgttcgaatt cgccaatgac aagacgctgg gcggggtttg
     2101 ctcgacattg ggtggaaaca ttccaggcct gggtggagag gctttttgct tcctcttgca
     2161 aaaccacact gctcgacatt gggtggaaac attccaggcc tgggtggaga ggctttttgc
     2221 ttcctcttga aaaccacact gctcgagggc ccttacggcc ggtcgactag atgaaggaga
     2281 gcctttctct ctgggcaaga gcggtgcaat ggtgtgtaaa ggtagctgag aagacgaaaa
     2341 gggcaagcat cttcctgcta ccaggctggg gaggcccagg cccacgaccc cgaggagagg
     2401 gaacgcaggg agactgaggt gacccttctt tcccccgggg cccggtcgtg tggttcggtg
     2461 tctcttttct gttggaccct taccttgacc caggcgctgc cggggcctgg gcccgggctg
     2521 cggcgcacgg cactcccggg aggcagcgag actcgagtta ggcccaacgc ggcgccacgg
     2581 cgtttcctgg ccgggaatgg cccgtacccg tgaggtgggg gtggggggca gaaaaggcgg
     2641 agcgagcccg aggcggggag ggggagggcc aggggcggag ggggccggca ctactgtgtt
     2701 ggcggactgg cgggactagg gctgcgtgag tctctgagcg caggcgggcg gcggccgccc
     2761 ctcccccggc ggcggcagcg gcggcagcgg cggcagctca ctcagcccgc tgcccgagcg
     2821 gaaacgccac tgaccgcacg gggattccca gtgccggcgc caggggcacg cgggacacgc
     2881 cccctcccgc cgcgccattg gcctctccgc ccaccgcccc acacttattg gccggtgcgc
     2941 cgccaatcag cggaggctgc cggggccgcc taaagaagag gctgtgcttt ggggctccgg
     3001 ctcctcagag agcctcggct aggtagggga tcgggactct ggcgggaggg cggcttggtg
     3061 cgtttgcggg gatgggcggc cgcggcaggc cctccgagcg tggtggagcc gttctgtgag
     3121 acagccgggt acgagtcgtg acgctggaag gggcaagcgg gtggtgggca ggaatgcggt
     3181 ccgccctgca gcaaccggag ggggagggag aagggagcgg aaaagtctcc accggacgcg
     3241 gccatggctc gggggggggg gggcagcgga ggagcgcttc cggccgacgt ctcgtcgctg
     3301 attggcttct tttcctcccg ccgtgtgtga aaacacaaat ggcgtgtttt ggttggcgta
     3361 aggcgcctgt cagttaacgg cagccggagt gcgcagccgc cggcagcctc gctctgccca
     3421 ctgggtgggg cgggaggtag gtggggtgag gcgagctgga cgtgcgggcg cggtcggcct
     3481 ctggcggggc gggggagggg agggagggtc agcgaaagta gctcgcgcgc gagcggccgc
     3541 ccaccctccc cttcctctgg gggagtcgtt ttacccgccg ccggccgggc ctcgtcgtct
     3601 gattggctct cggggcccag aaaactggcc cttgccattg gctcgtgttc gtgcaagttg
     3661 agtccatccg ccggccagcg ggggcggcga ggaggcgctc ccaggttccg gccctcccct
     3721 cggccccgcg ccgcagagtc tggccgcgcg cccctgcgca acgtggcagg aagcgcgcgc
     3781 tgggggcggg gacgggcagt agggctgagc ggctgcgggg cgggtgcaag cacgtttccg
     3841 acttgagttg cctcaagagg ggcgtgctga gccagacctc catcgcgcac tccggggagt
     3901 ggagggaagg agcgagggct cagttgggct gttttggagg caggaagcac ttgctctccc
     3961 aaagtcgctc tgagttgtta tcagtaaggg agctgcagtg gagtaggcgg ggagaaggcc
     4021 gcacccttct ccggaggggg gaggggagtg ttgcaatacc tttctgggag ttctctgctg
     4081 cctcctggct tctgaggacc gccctgggcc tgggagaatc ccttccccct cttccctcgt
     4141 gatctgcaac tccagtctga tatcaaaact ctcttaaggt agctagggcg cagtagtcca
     4201 gggtttcctt gatgatgtca tacttatcct gtcccttttt tttccacagc tcgcggttga
     4261 ggacaaactc ttcgcggtct ttccagtggg gatcgacggt atcgataagc tgaagttcct
     4321 attccgaagt tcctattcta tcagaagtat aggaacttcg tagtgcccca actggggtaa
     4381 cctttgagtt ctctcagttg ggggcgtaga attattcgta tagcatacat tatacgaagt
     4441 tataccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     4501 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     4561 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     4621 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     4681 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     4741 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     4801 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     4861 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     4921 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     4981 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     5041 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     5101 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     5161 cgagctgtac aagggcagcg gagagggcag aggaagtctg ctaacatgcg gtgacgtgga
     5221 ggagaatccc ggccccattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     5281 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     5341 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     5401 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     5461 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     5521 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     5581 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     5641 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     5701 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     5761 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     5821 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     5881 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     5941 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     6001 ctatcgcctt cttgacgagt tcttctgacc cagacctggc acccaggaga ggagcaggca
     6061 gggtcagctg cgccgcgact ctagatcata atcagccata ccacatttgt agaggtttta
     6121 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     6181 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6241 aatttcacaa ataaagcatt tttttcactg cgaagttcct atactttcta gagaatatga
     6301 acttcggaat aggaacttca taacttcgta taggatactt tatacgaagt tatgaagttc
     6361 ctatactcct ataagaatag gaacttcgga ataggaactt ctaactataa cggtcctaag
     6421 gtagcgatat cttctgggca ggcttaaagg ctaacctggt gtgtgggcgt tgtcctgcag
     6481 gggaattgaa caggtgtaaa attggaggga caagacttcc cacagatttt cggttttgtc
     6541 gggaagtttt ttaatagggg caaataagga aaatgggagg ataggtagtc atctggggtt
     6601 ttatgcagca aaactacagg ttattattgc ttgtgatccg cctcggagta ttttccatcg
     6661 aggtagatta aagacatgct cacccgagtt ttatactctc ctgcttgaga tccttactac
     6721 agtatgaaat tacagtgtcg cgagttagac tatgtaagca gaattttaat catttttaaa
     6781 gagcccagta cttcatatcc atttctcccg ctccttctgc agccttatca aaaggtattt
     6841 tagaacactc attttagccc cattttcatt tattatactg gcttatccaa cccctagaca
     6901 gagcattggc attttccctt tcctgatctt agaagtctga tgactcatga aaccagacag
     6961 attagttaca tacaccacaa atcgaggctg tagctggggc ctcaacactg cagttctttt
     7021 ataactcctt agtacacttt ttgttgatcc tttgccttga tccttaattt tcagtgtcta
     7081 tcacctctcc cgtcaggtgg tgttccacat ttgggcctat tctcagtcca gggagtttta
     7141 caacaataga tgtattgaga atccaaccta aagcttaact ttccactccc atgaatgcct
     7201 ctctcctttt tctccattta taaactgagc tattaaccat taatggtttc caggtggatg
     7261 tctcctcccc caatattacc tgatgtatct tacatattgc caggctgata ttttaagaca
     7321 ttaaaaggta tatttcatta ttgagccaca tggtattgat tactgcttac taaaattttg
     7381 tcattgtaca catctgtaaa aggtggttcc ttttggaatg caaagttcag gtgtttgttg
     7441 tctttcctga cctaaggtct tgtgagcttg tattttttct atttaagcag tgctttctct
     7501 tggactggct tgactcatgg cattctacac gttattgctg gtctaaatgt gattttgcca
     7561 agcttcttca ggacctataa ttttgcttga cttgtagcca aacacaagta aaatgattaa
     7621 gcaacaaatg tatttgtgaa gcttggtttt taggttgttg tgttgtgtgt gcttgtgctc
     7681 tataataata ctatccaggg gctggagagg tggctcggag ttcaagagca cagactgctc
     7741 ttccagaagt cctgagttca attcccagca accacatggt ggctcacaac catctgtaat
     7801 gggatctgat gccctcttct ggtgtgtctg aagaccacaa gtgtattcac attaaataaa
     7861 taaatcctcc ttcttcttct tttttttttt tttaaagaga atactgtctc cagtagaatt
     7921 tactgaagta atgaaatact ttgtgtttgt tccaatatgg tagccaataa tcaaattact
     7981 ctttaagcac tggaaatgtt accaaggaac taatttttat ttgaagtgta actgtggaca
     8041 gaggagccat aactgcagac ttgtgggata cagaagacca atgcagactt taatgtcttt
     8101 tctcttacac taagcaataa agaaataaaa attgaacttc tagtatccta tttgtttaaa
     8161 ctgctagctt tacttaactt ttgtgcttca tctatacaaa gctgaaagct aagtctgcag
     8221 ccattactaa acatgaaagc aagtaatgat aattttggat ttcaaaaatg tagggccaga
     8281 gtttagccag ccagtggtgg tgcttgcctt tatgccttta atcccagcac tctggaggca
     8341 gagacaggca gatctctgag tttgagccca gcctggtcta cacatcaagt tctatctagg
     8401 atagccagga atacacacag aaaccctgtt ggggaggggg gctctgagat ttcataaaat
     8461 tataattgaa gcattcccta atgagccact atggatgtgg ctaaatccgt ctacctttct
     8521 gatgagattt gggtattatt ttttctgtct ctgctgttgg ttgggtcttt tgacactgtg
     8581 ggctttcttt aaagcctcct tcctgccatg tggtctcttg tttgctacta acttcccatg
     8641 gcttaaatgg catggctttt tgccttctaa gggcagctgc tgagatttgc agcctgattt
     8701 ccagggtggg gttgggaaat ctttcaaaca ctaaaattgt cctttaattt tttttttaaa
     8761 aaatgggtta tataataaac ctcataaaat agttatgagg agtgaggtgg actaatatta
     8821 aatgagtccc tcccctataa aagagctatt aaggcttttt gtcttatact taactttttt
     8881 tttaaatgtg gtatctttag aaccaagggt cttagagttt tagtatacag aaactgttgc
     8941 atcgcttaat cagattttct agtttcaaat ccagagaatc caaattcttc acagccaaag
     9001 tcaaattaag aatttctgac ttttaatgtt aatttgctta ctgtgaatat aaaaatgata
     9061 gcttttcctg aggcagggtc tcactatgta tctctgcctg atctgcaaca agatatgtag
     9121 actaaagttc tgcctgcttt tgtctcctga atactaaggt taaaatgtag taatactttt
     9181 ggaacttgca ggtcagattc ttttataggg gacacactaa gggagcttgg gtgatagttg
     9241 gtaaaatgtg tttcaagtga tgaaaacttg aattattatc accgcaacct actttttaaa
     9301 aaaaaaagcc aggcctgtta gagcatgctt aagggatccc taggacttgc tgagcacaca
     9361 agagtagtta cttggcaggc tcctggtgag agcatatttc aaaaaacaag gcagacaacc
     9421 aagaaactac agttaaggtt acctgtcttt aaaccatctg catatacaca gggatattaa
     9481 aatattccaa ataatatttc attcaagttt tcccccatca aattgggaca tggatttctc
     9541 cggtgaatag gcagagttgg aaactaaaca aatgttggtt ttgtgatttg tgaaattgtt
     9601 ttcaagtgat agttaaagcc catgagatac agaacaaagc tgctatttcg aggtctcttg
     9661 gtttatactc agaagcactt ctttgggttt ccctgcacta tcctgatcat gtgctaggcc
     9721 taccttaggc tgattgttgt tcaaataaac ttaagtttcc tgtcaggtga tgtcatatga
     9781 tttcatatat caaggcaaaa catgttatat atgttaaaca tttgtactta atgtgaaagt
     9841 taggtctttg tgggtttgat ttttaatttt caaaacctga gctaaataag tcatttttac
     9901 atgtcttaca tttggtggaa ttgtataatt gtggtttgca ggcaagactc tctgacctag
     9961 taaccctacc tatagagcac tttgctgggt cacaagtcta ggagtcaagc atttcacctt
    10021 gaagttgaga cgttttgtta gtgtatacta gtttatatgt tggaggacat gtttatccag
    10081 aagatattca ggactatttt tgactgggct aaggaattga ttctgattag cactgttagt
    10141 gagcattgag tggcctttag gcttgaattg gagtcacttg tatatctcaa ataatgctgg
    10201 ccttttttaa aaagcccttg ttctttatca ccctgttttc tacataattt ttgttcaaag
    10261 aaatacttgt ttggatctcc ttttgacaac aatagcatgt tttcaagcca tatttttttt
    10321 cctttttttt tttttttttg gtttttcgag acagggtttc tctgtatagc cctggctgtc
    10381 ctggaactca ctttgtagac caggctggcc tcgaactcag aaatccgcct gcctctgcct
    10441 cctgagtgcc gggattaaag gcgtgcacca ccacgcctgg ctaagttgga tattttgtta
    10501 tataactata accaatacta actccactgg gtggattttt aattcagtca gtagtcttaa
    10561 gtggtcttta ttggcccttc attaaaatct actgttcact ctaacagagg ctgttggtac
    10621 tagtggcact taagcaactt cctacggata tactagcaga ttaagggtca gggatagaaa
    10681 ctagtctagc gttttgtata cctaccagct ttatactacc ttgttctgat agaaatattt
    10741 caggacatct agagtgtact ataaggttga tggtaagctt ataaggaact tgaaagtgga
    10801 gtaactactc catttctctg aggggagaat taaaattttt gaccaagtgt tgttgagcca
    10861 ctgagaatgg tctcagaaca taacttctta aggaaccttc ccagattgcc ctcaacactg
    10921 caccacattt ggtcctgctt gaacattgcc atggctctta aagtcttaat taagaatatt
    10981 aattgtgtaa ttattgtttt tcctccttta gatcattcct tgaggacagg acagtgcttg
    11041 tttaaggcta tatttctgct gtctgagcag caacaggtct tcgagatcaa catgatgttc
    11101 ataatcccaa gatgttgcca tttatgttct cagaagcaag cagaggcatg atggtcagtg
    11161 acggcgcgtg gcctccgagg ccattaccct gttatcccta cataggccca attgccacgg
    11221 cccggtaccc aattcgccct atagtgagtc gtattacgcg cgcttggcgt aatcatggtc
    11281 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    11341 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    11401 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    11461 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    11521 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    11581 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    11641 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    11701 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    11761 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    11821 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    11881 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    11941 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    12001 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    12061 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    12121 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    12181 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    12241 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    12301 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    12361 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    12421 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    12481 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    12541 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    12601 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    12661 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    12721 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    12781 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    12841 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    12901 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    12961 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    13021 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    13081 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    13141 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    13201 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    13261 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    13321 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    13381 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
