LOCUS       sequence-103265-       11299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for amiR-eGFP knock-in via landing pad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11299)
  AUTHORS   Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato
            M, Ohtsuka M
  TITLE     Assessment of Artificial MiRNA Architectures for Higher Knockdown 
            Efficiencies without the Undesired Effects in Mice.
  JOURNAL   PLoS One. 2015 Aug 18;10(8):e0135919. doi: 
            10.1371/journal.pone.0135919. eCollection 2015.
  PUBMED    26285215
REFERENCE   2  (bases 1 to 11299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-18"; volume: "10(8):e0135919. doi"; pages: " 
            10.1371/journal.pone.0135919. eCollection 2015"
COMMENT     Sequence Label: pAWV
FEATURES             Location/Qualifiers
     source          1..11299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(115..148)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     163..179
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     163..179
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    203..324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     ncRNA           complement(502..543)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(607..633)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(640..681)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(745..771)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     CDS             complement(831..2261)
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     regulatory      2258..2267
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          2335..3343
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     promoter        complement(3344..3619)
                     /label=chicken beta-actin promoter
     enhancer        3622..4001
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    complement(4016..4049)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(4060..4093)
                     /label=loxJTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4280..4297)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4507..4526
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     polyA_signal    4594..4715
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(4853..6124,6125..6145,6146..6151))
                     /codon_start=1
                     /product="nuclear-targeted site-specific recombinase"
                     /label=FLPe
                     /note="FLPe is a fusion between the SV40 nuclear 
                     localization signal and a thermostable version of the 
                     Saccharomyces cerevisiae site-specific recombinase FLP. 
                     Recombination occurs at FRT sequences (Buchholz et al., 
                     1998)."
                     /translation="MAPKKKRKVMSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAA
                     ELTYLCWMITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKK
                     LIPAWEFTIIPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESI
                     WEITEKILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGV
                     IIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEY
                     QLLKDNLVRSYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWS
                     DKRASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQL
                     KGSAEGSIRYPAWNGIISQEVLDYLSSYINRRI"
     regulatory      6148..6157
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          6225..7233
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     promoter        complement(7234..7509)
                     /label=chicken beta-actin promoter
     enhancer        7512..7815
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             8853..9044
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(9038..9060)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9146..9286
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9201..9220
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9301..9318)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9472..10060)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9552..9571)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10231..11022,11023..11091))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10854..10873
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11092..11196)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11264..11282
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcggtac caataaaaga gctttatttt catgatctgt
       61 gtgttggttt ttgtgtgcgg cgcgcgcgcc agcttggcgt ataactgcta gtacgaagtt
      121 cctatacttt ctagagaata ggaacttcgc tagagcggcc gctctagaac tagtggatca
      181 ttaacgctta caatttacgc cttaagatac attgatgagt ttggacaaac cacaactaga
      241 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
      301 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      361 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
      421 gattatgatc tagagtcgcg gccggccgct ttacttgtac aagaaagctg ggtctagata
      481 tctcgagtgc ggccagatct gggccatttg ttccatgtga gtgctagtaa caggccttgt
      541 gtcctgtgta gttgtaccag cttgtgtcag tcagtggcca aaacacaagc tggagtacaa
      601 ctacacagca tacagccttc agcaagcctc caggatctgg gccatttgtt ccatgtgagt
      661 gctagtaaca ggccttgtgt cctgatgaac ttcagtcagc ttggtcagtc agtggccaaa
      721 accaagctga ccctgaagtt catcagcata cagccttcag caagcctcca ggatccactg
      781 gtcgactcac tacctccctt taaacgatcg acggccacga agtgcttagc ttacttgtac
      841 agctcgtcca tgccgtacag gaacaggtgg tggcggccct cggagcgctc gtactgttcc
      901 acgatggtgt agtcctcgtt gtgggaggtg atgtccagct tggtgtccac gtagtagtag
      961 ccgggcagtt gcacgggctt cttggccatg tagatggtct tgaactccac caggtagtgg
     1021 ccgccgtcct tcagcttcag ggcctggtgg atctcgccct tcagcacgcc gtcgcggggg
     1081 tacaggcgct cggtggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg
     1141 ggggggaagt tggtgccgcg catcttcacc ttgtagatca gcgtgccgtc ctgcagggag
     1201 gagtcctggg tcacggtcac cagaccgccg tcctcgaagt tcatcacgcg ctcccacttg
     1261 aagccctcgg ggaaggacag cttcttgtaa tcggggatgt cggcggggtg cttcacgtac
     1321 gccttggagc cgtacatgaa ctggggggac aggatgtccc aggcgaaggg cagggggccg
     1381 cccttggtca ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg
     1441 ccctcgatct cgaactcgtg gccgttcatg gagccctcca tgcgcacctt gaagcgcatg
     1501 aactctttga tgacggccat gttgttgtcc tcggaggagg cggtgccgga gctgccgctg
     1561 ccggtgctgc cggtgccatg ccccaggaac aggtggtggc ggccctcgga gcgctcgtac
     1621 tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccagcttggt gtccacgtag
     1681 tagtagccgg gcagttgcac gggcttcttg gccatgtaga tggtcttgaa ctccaccagg
     1741 tagtggccgc cgtccttcag cttcagggcc tggtggatct cgcccttcag cacgccgtcg
     1801 cgggggtaca ggcgctcggt ggaggcctcc cagcccatgg tcttcttctg cattacgggg
     1861 ccgtcggggg ggaagttggt gccgcgcatc ttcaccttgt agatcagcgt gccgtcctgc
     1921 agggaggagt cctgggtcac ggtcaccaga ccgccgtcct cgaagttcat cacgcgctcc
     1981 cacttgaagc cctcggggaa ggacagcttc ttgtaatcgg ggatgtcggc ggggtgcttc
     2041 acgtacgcct tggagccgta catgaactgg ggggacagga tgtcccaggc gaagggcagg
     2101 gggccgccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg
     2161 ccctcgccct cgatctcgaa ctcgtggccg ttcatggagc cctccatgcg caccttgaag
     2221 cgcatgaact ctttgatgac ctcctcgccc ttgctcacca tggtggcgac cggtggatcc
     2281 caattctttg ccaaaatgat gagacagcac aacaaccagc acgttgccca ggagctgtag
     2341 gaaaaagaag aaggcatgaa catggttagc agaggctcta gagccgccgg tcacacgcca
     2401 gaagccgaac cccgccctgc cccgtccccc ccgaaggcag ccgtccccct gcggcagccc
     2461 cgaggctgga gatggagaag gggacggcgg cgcggcgacg cacgaaggcc ctccccgccc
     2521 atttccttcc tgccggcgcc gcaccgcttc gcccgcgccc gctagagggg gtgcggcggc
     2581 gcctcccaga tttcggctcc gccagatttg ggacaaagga agtccctgcg ccctctcgca
     2641 cgattaccat aaaaggcaat ggctgcggct cgccgcgcct cgacagccgc cggcgctccg
     2701 gggccgccgc gcccctcccc cgagccctcc ccggcccgag gcggccccgc cccgcccggc
     2761 acccccacct gccgccaccc cccgcccggc acggcgagcc ccgcgccacg ccccgcacgg
     2821 agccccgcac ccgaagccgg gccgtgctca gcaactcggg gaggggggtg cagggggggg
     2881 ttacagcccg accgccgcgc ccacaccccc tgctcacccc cccacgcaca caccccgcac
     2941 gcagcctttg ttcccctcgc agcccccccg caccgcgggg caccgccccc ggccgcgctc
     3001 ccctcgcgca cacgcggagc gcacaaagcc ccgcgccgcg cccgcagcgc tcacagccgc
     3061 cgggcagcgc gggccgcacg cggcgctccc cacgcacaca cacacgcacg caccccccga
     3121 gccgctcccc cccgcacaaa gggccctccc ggagcccttt aaggctttca cgcagccaca
     3181 gaaaagaaac gagccgtcat taaaccaagc gctaattaca gcccggagga gaagggccgt
     3241 cccgcccgct cacctgtggg agtaacgcgg tcagtcagag ccggggcggg cggcgcgagg
     3301 cggcgcggag cggggcacgg ggcgaaggca acgcagcgac tcccgcccgc cgcgcgcttc
     3361 gctttttata gggccgccgc cgccgccgcc tcgccataaa aggaaacttt cggagcgcgc
     3421 cgctctgatt ggctgccgcc gcacctctcc gcctcgcccc gccccgcccc tcgccccgcc
     3481 ccgccccgcc tggcgcgcgc cccccccccc cccgccccca tcgctgcaca aaataattaa
     3541 aaaataaata aatacaaaat tgggggtggg gagggggggg agatggggag agtgaagcag
     3601 aacgtggggc tcacctcgac ccatggtaat agcgatgact aatacgtaga tgtactgcca
     3661 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
     3721 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
     3781 agtttaccgt aaatagtcca cccattgacg tcaatggaaa gtccctattg gcgttactat
     3841 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
     3901 gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc
     3961 cgtaattgat tactattaat aactagtcaa taatcaatgt cgagcggccg ctagaataac
     4021 ttcgtatagg atactttata cgaagttatc tcctaggcaa taacttcgta tagcatacat
     4081 tatagcaatt tatgctagaa ttaagaattc cgcccctctc cctccccccc ccctaacgtt
     4141 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     4201 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     4261 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     4321 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     4381 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     4441 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     4501 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtacct
     4561 ctagtggatc attaacgctt acaatttacg ccttaagata cattgatgag tttggacaaa
     4621 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     4681 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     4741 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     4801 gtggtatggc tgattatgat ctagagtcgc ggccggccgc tttacttgta cattatatgc
     4861 gtctatttat gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg
     4921 cggggtatcg tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct
     4981 caattggatt agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatatgcat
     5041 agtaccgaga aactagtgcg aagtagtgat caggtattgc tgttatctga tgagtatacg
     5101 ttgtcctggc cacggcagaa gcacgcttat cgctccaatt tcccacaaca ttagtcaact
     5161 ccgttaggcc cttcattgac agaaatgagg tcatcaaatg tcttccaatg tgagattttg
     5221 ggccattctt tatagcaaag attggataag gcgcattttt cttcaaagcc ttgttgtacg
     5281 atctgactaa gttatctttt aataattggt attcctgttt gttgcttgaa gaattgccgg
     5341 tcctatttac tcgttttagg actggttcag aattcctcaa aaattcatcc aaatatacaa
     5401 gtggatcgat cctacccctt gcgctaaaga agtatatgtg cctactaacg cttgtctttg
     5461 tctctgtcac taaacactgg attattactc ccagatactt attttggact aatttaaatg
     5521 atttcggatc aacgttctta atatcgctga atcttccaca attgatgaaa gtagctagga
     5581 agaggaattg gtataaagtt tttgtttttg taaatctcga ggtatactca aacgaattta
     5641 gtattttctc agtgatctcc cagatgcttt caccctcact tagaagtgct ttaagcattt
     5701 ttttactgtg gctatttccc ttatctgctt cttccgatga ttcgaactgt aattgcaaac
     5761 tacttacaat atcagtgata tcagattgat gtttttgtcc attgtaagga ataattgtaa
     5821 attcccaagc aggaattaat ttctttaatg aggcttccag aattgttgct ttttgcgtct
     5881 tgtatttaaa ctggagtgat ttgttgacaa tatcgaaact cagcgaattg cttatgatag
     5941 tattatagct catgaatgtg gctctcttga ttgctgttcc gttatgagta atcatccaac
     6001 ataaataggt tagttcagca gcacatgatg ctattttttc ccctgaaggt ctttcaaacc
     6061 tttccacaaa ctgacgaacc aggaccttag gtggtgtttt acataatata tcaaattgac
     6121 tcatcacctt cctcttcttc ttgggagcca tggtggcgac cggtggatcc caattctttg
     6181 ccaaaatgat gagacagcac aacaaccagc acgttgccca ggagctgtag gaaaaagaag
     6241 aaggcatgaa catggttagc agaggctcta gagccgccgg tcacacgcca gaagccgaac
     6301 cccgccctgc cccgtccccc ccgaaggcag ccgtccccct gcggcagccc cgaggctgga
     6361 gatggagaag gggacggcgg cgcggcgacg cacgaaggcc ctccccgccc atttccttcc
     6421 tgccggcgcc gcaccgcttc gcccgcgccc gctagagggg gtgcggcggc gcctcccaga
     6481 tttcggctcc gccagatttg ggacaaagga agtccctgcg ccctctcgca cgattaccat
     6541 aaaaggcaat ggctgcggct cgccgcgcct cgacagccgc cggcgctccg gggccgccgc
     6601 gcccctcccc cgagccctcc ccggcccgag gcggccccgc cccgcccggc acccccacct
     6661 gccgccaccc cccgcccggc acggcgagcc ccgcgccacg ccccgcacgg agccccgcac
     6721 ccgaagccgg gccgtgctca gcaactcggg gaggggggtg cagggggggg ttacagcccg
     6781 accgccgcgc ccacaccccc tgctcacccc cccacgcaca caccccgcac gcagcctttg
     6841 ttcccctcgc agcccccccg caccgcgggg caccgccccc ggccgcgctc ccctcgcgca
     6901 cacgcggagc gcacaaagcc ccgcgccgcg cccgcagcgc tcacagccgc cgggcagcgc
     6961 gggccgcacg cggcgctccc cacgcacaca cacacgcacg caccccccga gccgctcccc
     7021 cccgcacaaa gggccctccc ggagcccttt aaggctttca cgcagccaca gaaaagaaac
     7081 gagccgtcat taaaccaagc gctaattaca gcccggagga gaagggccgt cccgcccgct
     7141 cacctgtggg agtaacgcgg tcagtcagag ccggggcggg cggcgcgagg cggcgcggag
     7201 cggggcacgg ggcgaaggca acgcagcgac tcccgcccgc cgcgcgcttc gctttttata
     7261 gggccgccgc cgccgccgcc tcgccataaa aggaaacttt cggagcgcgc cgctctgatt
     7321 ggctgccgcc gcacctctcc gcctcgcccc gccccgcccc tcgccccgcc ccgccccgcc
     7381 tggcgcgcgc cccccccccc cccgccccca tcgctgcaca aaataattaa aaaataaata
     7441 aatacaaaat tgggggtggg gagggggggg agatggggag agtgaagcag aacgtggggc
     7501 tcacctcgac ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
     7561 tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
     7621 atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
     7681 aaatagtcca cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac
     7741 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
     7801 gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat
     7861 tactattaat aactagaggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga
     7921 ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg
     7981 ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg
     8041 ctcgcggctc ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat
     8101 gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt
     8161 gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa
     8221 gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc
     8281 ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct
     8341 ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga
     8401 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga
     8461 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct
     8521 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
     8581 cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg
     8641 gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt
     8701 cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt
     8761 catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca
     8821 tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta
     8881 acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg
     8941 acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc
     9001 gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     9061 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     9121 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     9181 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     9241 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     9301 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     9361 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     9421 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     9481 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9541 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     9601 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     9661 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     9721 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     9781 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9841 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9901 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9961 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    10021 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    10081 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    10141 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    10201 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    10261 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    10321 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    10381 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    10441 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    10501 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt
    10561 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    10621 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    10681 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    10741 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    10801 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
    10861 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10921 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10981 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    11041 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    11101 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    11161 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    11221 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    11281 gccctttcgt cttcaagaa
//
