LOCUS       sequence-103270-         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human p53 (1-73) (P12A, P13A, P27A) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Borcherds W, Theillet FX, Katzer A, Finzel A, Mishall KM, Powell AT,
            Wu H, Manieri W, Dieterich C, Selenko P, Loewer A, Daughdrill GW
  TITLE     Disorder and residual helicity alter p53-Mdm2 binding affinity and 
            signaling in cells.
  JOURNAL   Nat Chem Biol. 2014 Dec;10(12):1000-2. doi: 10.1038/nchembio.1668. 
            Epub 2014 Nov 2.
  PUBMED    25362358
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1668"; journalName: "Nat Chem Biol"; date: 
            "2014-12"; volume: "10"; issue: "12"; pages: "1000-2"
COMMENT     Sequence Label: pET28A-p53 (1-73) (PPP)
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(215..232)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(242..259)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             278..300
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    315..339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(339..358)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(340..358)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(442..461)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cagcctctgg cattctggga gcttcatctg gacctgggtc ttcagtgaac cattgttcaa
       61 tatcgtccgg ggacagcatc aaatcatcca ttgcttggga cggcaagggg gacagaacgt
      121 tgttttcagc aagtagtttc cataggtctg aaaatgtttc ctgactcaga gcggcctcga
      181 cgctaggatc tgactgcggc tcctccatca tatggctgcc gcgcggcacc aggccgctgc
      241 tgtgatgatg atgatgatgg ctgctgccca tggtatatct ccttcttaaa gttaaacaaa
      301 attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag tcgtattaat
      361 ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg
      421 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
      481 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
      541 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
      601 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag
      661 atcccggaca ccatcgaatg gcgc
//
