LOCUS       sequence-103273-         578 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human p53 (1-73) (all P to A) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Borcherds W, Theillet FX, Katzer A, Finzel A, Mishall KM, Powell AT,
            Wu H, Manieri W, Dieterich C, Selenko P, Loewer A, Daughdrill GW
  TITLE     Disorder and residual helicity alter p53-Mdm2 binding affinity and 
            signaling in cells.
  JOURNAL   Nat Chem Biol. 2014 Dec;10(12):1000-2. doi: 10.1038/nchembio.1668. 
            Epub 2014 Nov 2.
  PUBMED    25362358
REFERENCE   2  (bases 1 to 578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1668"; journalName: "Nat Chem Biol"; date: 
            "2014-12"; volume: "10"; issue: "12"; pages: "1000-2"
COMMENT     Sequence Label: pET28A-p53 (1-73) (P to A)
FEATURES             Location/Qualifiers
     source          1..578
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..28
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             260..277
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(330..348)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      344..391
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(515..534)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cagcagcggc ctggtgccgc gcggcagcca tatggaagaa gcccaatcag acgcctcagt
       61 tgaagccgcc ttaagccaag aaaccttttc agatttatgg aaattactcg ccgaaaataa
      121 tgtactctcc gccctcgcct ctcaagctat ggacgactta atgctttcag ccgatgatat
      181 tgaacaatgg tttaccgaag atgccggagc cgatgaagcc gcccgtatgg ccgaagccgc
      241 cgctgctgtt taactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc
      301 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
      361 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc
      421 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      481 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      541 ctcgccacgt tcgccggctt tccccgtcaa gctctaaa
//
