LOCUS       sequence-103276-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Borcherds W, Theillet FX, Katzer A, Finzel A, Mishall KM, Powell AT,
            Wu H, Manieri W, Dieterich C, Selenko P, Loewer A, Daughdrill GW
  TITLE     Disorder and residual helicity alter p53-Mdm2 binding affinity and 
            signaling in cells.
  JOURNAL   Nat Chem Biol. 2014 Dec;10(12):1000-2. doi: 10.1038/nchembio.1668. 
            Epub 2014 Nov 2.
  PUBMED    25362358
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1668"; journalName: "Nat Chem Biol"; date: 
            "2014-12"; volume: "10"; issue: "12"; pages: "1000-2"
COMMENT     Sequence Label: pET28A-p53 (1-73) (P12A P13A)
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(216..233)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(243..260)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             279..301
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    316..340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(340..359)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(341..359)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(443..462)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cagcctctgg cattctggga gcttcatctg gacctgggtc ttcagatgaa ccattgttca
       61 atatcgtccg gggacagcat caaatcatcc attgcttggg acggcaaggg ggacagaacg
      121 ttgttttcag gaagtagttt ccataggtct gaaaatgttt cctgactcag agcggcctcg
      181 acgctaggat ctgactgcgg ctcctccatc atatggctgc cgcgcggcac caggccgctg
      241 ctgtgatgat gatgatgatg gctgctgccc atggtatatc tccttcttaa agttaaacaa
      301 aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa
      361 tttcgcggga tcgagatctc gatcctctac gccggacgca tcgtggccgg catcaccggc
      421 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
      481 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
      541 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
      601 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
      661 gatcccggac accatcgaat ggcgcaaaac ctttcgcggt atggcatgat agcgcccgga
      721 agaga
//
