LOCUS       sequence-103278-         651 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To construct C-terminal GFP fusions, single crossover integration 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Eberhardt A, Wu LJ, Errington J, Vollmer W, Veening JW
  TITLE     Cellular localization of choline-utilization proteins in 
            Streptococcus pneumoniae using novel fluorescent reporter systems.
  JOURNAL   Mol Microbiol. 2009 Oct;74(2):395-408. doi: 
            10.1111/j.1365-2958.2009.06872.x. Epub 2009 Sep 8.
  PUBMED    19737355
REFERENCE   2  (bases 1 to 651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2958.2009.06872.x"; journalName: "Mol Microbiol"; 
            date: "2009-10"; volume: "74"; issue: "2"; pages: "395-408"
COMMENT     Sequence Label: pAE03
FEATURES             Location/Qualifiers
     source          1..651
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(200..220)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tttttttgtt tttataaatc atgatatgtc ttagatttta ctagttaaat acgcttcaca
       61 gtttcttctt catttttaac aaacaaaaaa cccagctcat tgagctgggt ttaagctggc
      121 ttggcggcgt cctaggatct ttattggccg gccttattat ttgtagagct catccatgcc
      181 atgtgtaatc ccagcagcag ttacaaactc aagaaggacc atgtggtcac gcttttcgtt
      241 gggatctttc gaaagggcag attgtgtcga caggtaatgg ttgtctggta aaaggacagg
      301 gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgg atccatcttc
      361 aatgttgtgg cgaattttga agttagcttt gattccattc ttttgtttgt ctgccgtgat
      421 gtatacattg tgtgagttat agttgtactc gagtttgtgt ccgagaatgt ttccatcttc
      481 tttaaaatca atacctttta actcgatacg attaacaagg gtatcacctt caaacttgac
      541 ttcagcacgc gtcttgtagt tcccgtcatc tttgaaagat atagtgcgtt cctgtacata
      601 accttcgggc atggcactct tgaaaaagtc atgccgtttc atatgatccg g
//
