LOCUS       sequence-103279-        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To construct C-terminal GFP fusions, single crossover integration 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Eberhardt A, Wu LJ, Errington J, Vollmer W, Veening JW
  TITLE     Cellular localization of choline-utilization proteins in 
            Streptococcus pneumoniae using novel fluorescent reporter systems.
  JOURNAL   Mol Microbiol. 2009 Oct;74(2):395-408. doi: 
            10.1111/j.1365-2958.2009.06872.x. Epub 2009 Sep 8.
  PUBMED    19737355
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2958.2009.06872.x"; journalName: "Mol Microbiol"; 
            date: "2009-10"; volume: "74"; issue: "2"; pages: "395-408"
COMMENT     Sequence Label: pAE03
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     575..603
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     misc_feature    642..698
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(711..727)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(711..727)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(724..746)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    735..751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(759..765,766..783,784..789))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    804..825
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gggatctttc gaaagggcag attgtgtcga caggtaatgg ttgtctggta aaaggacagg
       61 gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgg atccatcttc
      121 aatgttgtgg cgaattttga agttagcttt gattccattc ttttgtttgt ctgccgtgat
      181 gtatacattg tgtgagttat agttgtactc gagtttgtgt ccgagaatgt ttccatcttc
      241 tttaaaatca atacctttta actcgatacg attaacaagg gtatcacctt caaacttgac
      301 ttcagcacgc gtcttgtagt tcccgtcatc tttgaaagat atagtgcgtt cctgtacata
      361 accttcgggc atggcactct tgaaaaagtc atgccgtttc atatgatccg gataacggga
      421 aaagcattga acaccatagg tcaaagtagt gacaagtgtt ggccatggaa caggtagttt
      481 tccagtagtg caaataaatt taagggtaag ctttccgtat gtagcatcac cttcaccctc
      541 tccactgaca gaaaatttgt gcccattaac atcaccatct aattcaacaa gaattgggac
      601 aactccagtg aaaagttctt ctcctttgct agcgcggccg cgaattcgag ctcggtaccc
      661 ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg
      721 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      781 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      841 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      901 gcggggagag gcggtttgcg tattggagct tgttgtaact gaaaaaggaa aattattgtg
      961 ccaggcagtt gaaagtcagc accttttaac gagtgctgaa atgacggcta aatgggaaac
     1021 gtatttaaaa aaa
//
