LOCUS       sequence-103292-        1265 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Talin/Focal Adhesions, C1 Cloning Vector, 
            Excitation:568, Emission:592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1265)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Talin-C-18
FEATURES             Location/Qualifiers
     source          1..1265
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             45..752
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     458..477
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      801..810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcaagctagt gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg
       61 aggagaataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
      121 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgaggcct
      181 ttcagaccgc taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
      241 tgtcccctca gttcatgtac ggctccaagg tctacattaa gcacccagcc gacatccccg
      301 actacttcaa gctgtccttc cccgagggct tcaggtggga gcgcgtgatg aacttcgagg
      361 acggcggcat tattcacgtt aaccaggact cctccctgca ggacggcgtg ttcatctaca
      421 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
      481 tgggctggga ggcctccgag gagcggatgt accccgagga cggcgccctg aagagcgaga
      541 tcaagaagag gctgaagctg aaggacggcg gccactacgc cgccgaggtc aagaccacct
      601 acaaggccaa gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca
      661 tcgtgtccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
      721 actccaccgg cggcatggac gagctgtaca agtccggact cagatctcga gctcaagcta
      781 gcttcgaatt cgcagaagct gccaccatgg ttgcgctttc gctgaagatt agcattggga
      841 atgtggtgaa gacgatgcaa tttgagccat ctaccatggt gtacgacgcc tgccgcatga
      901 ttcgtgagcg gatcccagag gccctggctg gccctcccaa cgactttggg ctctttctgt
      961 cagatgatga ccccaaaaag gcatctggct agaggctggg aaagctttgg actactacat
     1021 gctccgaaat ggggacacca tggagtacag aaaaagcaga gacccctgaa gaatacgatg
     1081 ttagacgacg tgagactatc atgtgatgac tccagacgtc acagacatgc ctcatgacat
     1141 ttgtgcccga atgattcacc acatgatgag aattcacttt tcggagcgtg tatggagaag
     1201 aaaggtacga ctggactggc agccagacct cactctgcgg agtaagaaat tggagacctc
     1261 atagc
//
