LOCUS       sequence-103294-        1243 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Talin Head Domain, H, Excitation:568, Emission:592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1243)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Talin-H-18
FEATURES             Location/Qualifiers
     source          1..1243
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             47..754
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     460..479
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      803..812
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtcagtgcca gtgacgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg
       61 cgaggagaat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
      121 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggc
      181 ctttcagacc gctaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
      241 cctgtcccct cagttcatgt acggctccaa ggtctacatt aagcacccag ccgacatccc
      301 cgactacttc aagctgtcct tccccgaggg cttcaggtgg gagcgcgtga tgaacttcga
      361 ggacggcggc attattcacg ttaaccagga ctcctccctg caggacggcg tgttcatcta
      421 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
      481 catgggctgg gaggcctccg aggagcggat gtaccccgag gacggcgccc tgaagagcga
      541 gatcaagaag aggctgaagc tgaaggacgg cggccactac gccgccgagg tcaagaccac
      601 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac atcgtcgaca tcaagttgga
      661 catcgtgtcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
      721 ccactccacc ggcggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc
      781 tagcttcgaa ttcgcagaag ctgccaccat ggttgcgctt tcgctgaaga ttagcattgg
      841 gaatgtggtg aagacgatgc aatttgagcc atctaccatg gtgtacgacg cctgccgcat
      901 gattcgtgag cggatcccag aggccctggc tgggccctcc caacgacttt gggctctttc
      961 tgtcagatga tgaccccaaa aaggcatctg gctagaggct ggggaaagct ttggactact
     1021 acatgctccg aaatggggac accatgagta cagaaaaaag cagagacccc tgagatcgat
     1081 gtagacgacg tgagactatc atgtgatgac tcagacgtca caagactgct catgaacatt
     1141 ttgtgccgat ggaatcacca acatgaatga gtattcactg gcttcgagag ctgatgagaa
     1201 gcaggatgag ggacggaatc cctgtgcaaa gacaggaccc tac
//
