LOCUS       sequence-103300-        1251 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, N1 Cloning Vector, Excitation:587, 
            Emission:610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1251)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-CenpA-N-22
FEATURES             Location/Qualifiers
     source          1..1251
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    453..505
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      513..522
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(664..682)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     932..951
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccatgctagt gacgtcagat ccgctagcca ccatgggccc gcgccgccgg agccgaaagc
       61 ccgaggcccc gaggaggcgc agcccgagcc cgaccccgac ccccggcccc tcccggcggg
      121 gcccctcctt aggcgcttcc tcccatcaac acagtcggcg gagacaaggt tggctaaagg
      181 agatccgaaa gcttcagaag agcacacacc tcttgataag gaagctgccc ttcagccgcc
      241 tggcaagaga aatatgtgtt aaattcactc gtggtgtgga cttcaattgg caagcccagg
      301 ccctattggc cctacaagag gcagcagaag catttctagt tcatctcttt gaggacgcct
      361 atctcctcac cttacatgca ggccgagtta ctctcttccc aaaggatgtg caactggccc
      421 ggaggatccg gggccttgag gagggactcg gcctcgagct caagcttcga attctgcagt
      481 cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg
      541 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
      601 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      661 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
      721 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      781 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      841 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
      901 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
      961 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaaggcg agatcaagca
     1021 gagctgaagc tgaagacgcg cactacgacg ctgagtcaga cactacaggc agagccgtgc
     1081 agctgcccgc gctacacgtc acatcagtga catcacctcc acacgaggac tactcatcgt
     1141 gcaacagtac gacgcgcgag gacgccactc cacggcgcat gacgactgaa agtacgccgc
     1201 acctgatcta tcagccataa cacattgtat gaaggttaac ctgtgccttt t
//
