LOCUS       sequence-103301-        1222 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, N1 Cloning Vector, Excitation:587, 
            Emission:610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1222)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-CenpA-N-22
FEATURES             Location/Qualifiers
     source          1..1222
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    388..440
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      448..457
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             454..1164
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(599..617)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     867..886
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tagaccgggg cggccggatc gaagtcgagt cctaggaggc agtcgatacg acccgaactc
       61 gacccttcgc gaggccctct tagcgctctc ccatccacac agtcgcgaga caagtgctaa
      121 agagatccga agcttcagag agcacacacc tctgataaga agctgccttc agccgctgca
      181 agaagaaata tgtgttaaat tcactcgtgt gtggacttca attggcaagc ccaggcccta
      241 ttggccctac aagaggcagc agaagcattt ctagttcatc tctttgagga cgcctatctc
      301 ctcactttac atgcaggccg agttactctc ttcccaaagg atgtgcaact ggcccggagg
      361 atccggggcc ttgaggaggg actcggcctc gagctcaagc ttcgaattct gcagtcgacg
      421 gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga ggaggataac
      481 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      541 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      601 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      661 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
      721 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      781 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      841 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      901 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
      961 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     1021 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     1081 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     1141 ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca
     1201 acattggtaa ggagtgatcc tg
//
