LOCUS       sequence-103302-        1300 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Bundling, C1 Cloning Vector, Excitation:587, 
            Emission:610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1300)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Espin-Like-ARD-C-18
FEATURES             Location/Qualifiers
     source          1..1300
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..753
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(191..209)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     459..478
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cccatgctag tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
       61 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      121 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      181 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      241 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      301 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      361 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      421 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      481 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
      541 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
      601 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
      661 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
      721 cactccaccg gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct
      781 tcgaattccg gtggcagcgg cgggagtatg gaggcgcagc aggcactggt ggcctccaag
      841 gatggggata tggccacctt ggaacggcta tttgaggctg gtgccctaag accagacatc
      901 actgatgatc tgggggctgg cctggtgcat catgccaccc gggctggtca cctggactgt
      961 gtcaagttcc tggtacagag agccaagctg ccaggcaacc agcagcacac aacggggcca
     1021 caccagtgca cgatgcagca gccacgggca cctggcagag ctgtgctggc tggtccgtga
     1081 tgcggctgcg gctgcagaac aagatgcctc aagtgttccc tccgcctgca ctgctgccgt
     1141 tggacaccaa gcctggtgga gtgttatctc tggagcatgc agtaccactg ggagcgtgaa
     1201 ggctaacact gcacacctgc gtaatggact gactgttctg atactctcgc agccacatgt
     1261 gtaacccgca gcagtgctca ttactgctgt ccaaagaacg
//
