LOCUS       sequence-103311-        1225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Kinesin Light Chain , N1 Cloning Vector, 
            Excitation:587, Emission:610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1225)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-KLC2-N-18
FEATURES             Location/Qualifiers
     source          1..1225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      457..466
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             463..1173
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(608..626)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     876..895
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cttggaattc ctggccagtc aactcaggtg tagaacggta aagatgcagt gtgaccgaga
       61 acaccgccgt ggcaggcaga gaatggtggc tggctcatct gagtccgact ggagggagtc
      121 agggctgcag ctgagtggag tggggacggc agcggctctc tgcggcgcag tggctccttg
      181 ggaagctccg ggatgctctg agacgcagca gtgagatgct ggtgaggaag ctgcagggag
      241 ggggccccac caggagcccc taactctagg atgaagaggg ccagctctct taacttcctt
      301 aacaagagtg tggaagagcc agtccagcct ggaggcacgg gtctttctga cagccgcact
      361 ttgagctcca gctccatgga cctctctcga agaagctccc tggtgggcgg cagcgcaggc
      421 ggaagcgggg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
      481 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
      541 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      601 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
      661 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
      721 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
      781 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
      841 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
      901 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
      961 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     1021 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     1081 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     1141 tccaccggcg gcatggacga gctgtacaag taaagcggcc gcgactctag atcataatca
     1201 gccataccac attgtagagt ggcac
//
