LOCUS       sequence-103313-        1219 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Kinesin Light Chain , N1 Cloning Vector, 
            Excitation:587, Emission:610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1219)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-KLC3-N-18
FEATURES             Location/Qualifiers
     source          1..1219
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      453..462
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             459..1169
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(604..622)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     872..891
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ttgtggccaa agagacttaa ggaacaacct gcttcaggcc tactgaagcg atcagattca
       61 gccagcagag gagctatcat ggagaatctc agtcagagct ggccggcccc acttgagccc
      121 ccccagggag gcacagcagc gataacaaca gcagcaggtc tgcgcagagc agttccttct
      181 ttaagctccg ggaatccatc aggcgaggaa gcgagaagct ggtctcccgc ctccgaggag
      241 aggggcatgg caggggctgc cgggatgaag agagccatgt cactcaatat gctgaacgtg
      301 gatggtccca gggctgccgg gacgcagctc tcccagctct caacccggca ctgagtgagg
      361 cccctcggac cctgagcatc agcacacaag atctgagccc gcgcggcagc gcaggcggaa
      421 gcggggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg
      481 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
      541 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      601 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
      661 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      721 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      781 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
      841 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
      901 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
      961 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     1021 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     1081 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     1141 ccggcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca
     1201 taccacattg tagagtgcc
//
