LOCUS       sequence-103325-        1249 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubule, N1 Cloning Vector, Excitation:487, 
            Emission:509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1249)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Enconsin-N-18
FEATURES             Location/Qualifiers
     source          1..1249
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      471..480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(477..479,480..482,483..1196)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(522..543)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(783..802)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1130..1151
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcgacccttg aggagagtat ggaaggatca ggaatcgagt cagatacgag caggatcagg
       61 aatcgtgaat aaggcaagga gcttccactg agaacagagg gtctgcattg catgtacaac
      121 aaaccgtccg ggaaatgaaa gcaggtgtag cccaccatgt gtactcacac agtcaaaagt
      181 gacagtggag gagcactccc gatttgaaaa caaccaaatg aaaatggtgt atctgtcaga
      241 atgaaaattt tgaagaaatt ataaacttac ccattggatc taaaccatcc agattagatg
      301 tcaccaacag tgagagccca gaaattcctt tgaatccaat tttggccttt gatgatgaag
      361 ggacacttgg gcccctgcct caggtagatg gtgttcagac acagcagact gcagaagtta
      421 taggcagcgc aggcggaagc ggggtaccgc gggcccggga tccaccggtc gccaccatgg
      481 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      541 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      601 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      661 tgaccacctt gacctacggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc
      721 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      781 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      841 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
      901 tggagtacaa ctacaacagc cacaaggtct atatcaccgc cgacaagcag aagaacggca
      961 tcaaggtgaa cttcaagacc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     1021 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1081 tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1141 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagcgg
     1201 ccgcgactct agatcataat cagccatacc acattgtaga gatttgcac
//
