LOCUS       sequence-103342-        1327 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Talin/Focal Adhesions, C1 Cloning Vector, 
            Excitation:487, Emission:509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1327)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Talin-C-18
FEATURES             Location/Qualifiers
     source          1..1327
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(45..47,48..50,51..761)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(90..111)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(351..370)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     698..719
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      810..819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcatgcttgt gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg
       61 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      121 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      181 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga
      241 cctacggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
      301 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      361 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      421 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      481 acaacagcca caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact
      541 tcaagacccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
      601 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
      661 ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
      721 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag
      781 ctcaagctag cttcgaattc gcagaagctg ccaccatggt tgcgctttcg ctgaagatta
      841 gcattgggaa tgtggtgaag acgatgcaat ttgagccatc taccatggtg tacgacgcct
      901 gccgcatgat tcgtgagcgg atcccagagg ccctggctgg ccctcccaac gactttgggc
      961 tctttctgtc agatgatgac cccaaaaagc atctggctag aggctgggaa agctttggga
     1021 ctactacatg ctccgaaatg gggacaccat ggagtacaga aaaagcagag accctgaaga
     1081 aacgatgtta gacgacgtga gactatcatg gtggatgact ccagaccgtc acagactgct
     1141 catgacattt gtgcccgaat tgtttcacca ccatgatgat attcactggt tcggagagcg
     1201 tgatgaagaa aagaaagaat gaggacggga cctgtgaaag acagacctat ctggcggatt
     1261 aaagaaatgg gagagctagc cgaaacgttg tccataccca acagatgaga gtggtatact
     1321 ctgccta
//
