LOCUS       sequence-103344-        1210 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, C1 Cloning Vector, Excitation:487, 
            Emission:509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1210)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-TPX2-M1-C-18
FEATURES             Location/Qualifiers
     source          1..1210
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(47..49,50..52,53..763)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(92..113)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(353..372)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     700..721
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcgattgcta gtgacgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg
       61 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      121 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      181 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
      241 gacctacggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
      301 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      361 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      421 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      481 ctacaacagc cacaaggtct atatcaccgc cgacaagcag aagaacggca tcaaggtgaa
      541 cttcaagacc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
      601 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
      661 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
      721 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatctcg
      781 agctcaagct tcgggagata gtggtgggag cggttcatca caagttaaaa gctcttattc
      841 ctatgatgcc ccctcggatt tcatcaattt ttcatccttg gatgatgaaa ggagatactc
      901 aaaacataga ttcatggttt gaggagaagg ccaatttgga gaataagtta ctggggagat
      961 ggaactggag ggctttttca ggcaaactcc tttgagaaag gctatcttca gcagctattg
     1021 tcacaccttt gaaacagtga cacacttact acaaagagca gaaagaaaat ctggtggaac
     1081 atccattcgt caatgctgtc tcctgagtga gccagcatat cagaaaactc ccagccccag
     1141 cctcgagaag atctcttagc tccggctaga ttggaacgag aagcctcatg tcaatgatag
     1201 gccagagaag
//
