LOCUS       sequence-103352-        1233 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton , N1 Cloning Vector, Excitation:505 / 
            569, Emission:516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1233)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-MYR-N-5
FEATURES             Location/Qualifiers
     source          1..1233
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      68..77
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             74..94
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             116..796
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gtcatgctag tgacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt
       61 cgaattcgcc accatgggaa gcagcaagag caagccaaag ggaccggtcg ccaccatgag
      121 tgcgattaag ccagacatga agatcaaact ccgtatggaa ggcaacgtaa acgggcacca
      181 ctttgtgatc gacggagatg gtacaggcaa gccttttgag ggaaaacaga gtatggatct
      241 tgaagtcaaa gagggcggac ctctgccttt tgcctttgat atcctgacca ctgcattcca
      301 ttacggcaac agggtattcg ccaaatatcc agacaacata caagactatt ttaagcagtc
      361 gtttcctaag gggtattcgt gggaacgaag cttgactttc gaagacgggg gcatttgcat
      421 tgccagaaac gacataacaa tggaagggga cactttctat aataaagttc gattttatgg
      481 taccaacttt cccgccaatg gtccagttat gcagaagaag acgctgaaat gggagccctc
      541 cactgagaaa atgtatgtgc gtgatggagt gctgacgggt gatattcata tggctttgtt
      601 gcttgaagga aatgcccatt accgatgtga cttcagaact acttacaaag ctaaggagaa
      661 gggtgtcaag ttaccaggct accactttgt ggaccactgc attgagattt taagccatga
      721 caaagattac aacaaggtta agctgtatga gcatgctgtt gctcattctg gattgcctga
      781 caatgccaga cgataagcgg ccgcgactct agatcataat cagccatacc acatttgtag
      841 aggttttact tgctttaaaa acctcccaca cctccccctg aacctgaaca taaatgaatg
      901 caattgtgtg ttacttgttt attgcagcta tatggtacaa taaagcatag catcacaaat
      961 tcacaataag cattttttca ctgcatctag tgtggttgtc caactcatca tgtatcttag
     1021 cgtaaatgta gcgttaatat ttgttaaatc gcgtaatttt gtaaatcagc tcattttaca
     1081 tagcgaatcg gcaaatcctt aatccaagaa tgaccggata ggggtggagt gggtggtcgt
     1141 tggaccgagt ccattaagac ctgatccacg tcaggcatcg ctctaccgat gcacttgaac
     1201 tgtcttcagt ttggtgcagt cctagctatc gac
//
