LOCUS       sequence-103353-        1245 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton , N1 Cloning Vector, Excitation:505 / 
            569, Emission:516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1245)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-MYR-N-5
FEATURES             Location/Qualifiers
     source          1..1245
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        349..400
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     351..371
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      467..476
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             473..493
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             515..1195
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 acggtcccgg catgagctcc gatgcgattc catgagccat gactcatgct catgggtgaa
       61 ttccggtact gccactggca gtactcaggt tatcatggca agtacgcccc tatgacgtca
      121 tgacgtaatg gccgctgcat atgccagtac atgactatgg actttctact ggcagtacat
      181 ctacgtatag tcatcgctat accatggtga tgcgtttgca gtacatcatg ggcgtggata
      241 gcggtttgac tcacgggatt tccaagtctc caccccatga cgtcaatggg agtttgtttg
      301 gcaccaaatc aacggacttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      361 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
      421 ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcgcca ccatgggaag
      481 cagcaagagc aagccaaagg gaccggtcgc caccatgagt gcgattaagc cagacatgaa
      541 gatcaaactc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg acggagatgg
      601 tacaggcaag ccttttgagg gaaaacagag tatggatctt gaagtcaaag agggcggacc
      661 tctgcctttt gcctttgata tcctgaccac tgcattccat tacggcaaca gggtattcgc
      721 caaatatcca gacaacatac aagactattt taagcagtcg tttcctaagg ggtattcgtg
      781 ggaacgaagc ttgactttcg aagacggggg catttgcatt gccagaaacg acataacaat
      841 ggaaggggac actttctata ataaagttcg attttatggt accaactttc ccgccaatgg
      901 tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa tgtatgtgcg
      961 tgatggagtg ctgacgggtg atattcatat ggctttgttg cttgaaggaa atgcccatta
     1021 ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt taccaggcta
     1081 ccactttgtg gaccactgca ttgagatttt aagccatgac aaagattaca acaaggttaa
     1141 gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac gataagcggc
     1201 cgcgactcta gatcataatc agccatacca cattgtagac ggttt
//
