LOCUS       sequence-103354-        1243 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton , N1 Cloning Vector, Excitation:507 / 
            572, Emission:516 / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1243)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos3.2-MYR-N-5
FEATURES             Location/Qualifiers
     source          1..1243
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      67..76
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             73..93
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             115..795
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    917..997
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gcatgctagt gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc
       61 gaattcgcca ccatgggaag cagcaagagc aagccaaagg gaccggtcgc caccatgagt
      121 gcgattaagc cagacatgaa gatcaaactc cgtatggaag gcaacgtaaa cgggcaccac
      181 tttgtgatcg acggagatgg tacaggcaag ccttttgagg gaaaacagag tatggatctt
      241 gaagtcaaag agggcggacc tctgcctttt gcctttgata tcctgaccac tgcattccat
      301 tacggcaaca gggtattcgc caaatatcca gacaacatac aagactattt taagcagtcg
      361 tttcctaagg ggtattcgtg ggaacgaagc ttgactttcg aagacggggg catttgcaac
      421 gccagaaacg acataacaat ggaaggggac actttctata ataaagttcg attttatggt
      481 accaactttc ccgccaatgg tccagttatg cagaagaaga cgctgaaatg ggagccctcc
      541 actgagaaaa tgtatgtgcg tgatggagtg ctgacgggtg atattgagat ggctttgttg
      601 cttgaaggaa atgcccatta ccgatgtgac ttcagaacta cttacaaagc taaggagaag
      661 ggtgtcaagt taccaggcgc ccactttgtg gaccactgca ttgagatttt aagccatgac
      721 aaagattaca acaaggttaa gctgtatgag catgctgttg ctcattctgg attgcctgac
      781 aatgccagac gataagcggc cgcgactcta gatcataatc agccatacca catttgtaga
      841 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
      901 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcatagc
      961 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagtgtgtt tgtcaaactc
     1021 atcatgtatc tatgcgtaaa tgtagcgtat atttgtaaat cgcgtaaatt ttgtaaatca
     1081 gctcattttt acaatagcga atcgccaatc ccttataatc aaagatagac ggataggtga
     1141 tgtgttcagt ggaacaagag tccattagac tgatccacgt caaggccaac gctactcagc
     1201 gatgcattga ctcctatcag ttgtgccagg tgctagccta ttc
//
