LOCUS       sequence-103360-        1237 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, C1 Cloning Vector, Excitation:505 / 569, 
            Emission:516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1237)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4a-CenpA-C-22
FEATURES             Location/Qualifiers
     source          1..1237
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             47..727
                     /codon_start=1
                     /product="fixation-resistant green-to-red photoswitchable 
                     fluorescent protein (Paez-Segala et al., 2015)"
                     /label=mEos4a
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICYA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 gccattctta gtgacgtcag atccgctagc gctaccggtc gccaccatgg tgagtgcgat
       61 taagccagac atgaggatca aactccgtat ggaaggcaac gtaaacgggc accactttgt
      121 gatcgacgga gatggtacag gcaagcctta tgagggaaaa cagaccatgg atcttgaagt
      181 caaagagggc ggacctctgc cttttgcctt tgatatcctg accactgcat tccattacgg
      241 caacagggta ttcgttaaat atccagacaa catacaagac tattttaagc agtcgtttcc
      301 taaggggtat tcgtgggaac gaagcttgac tttcgaagac gggggcattt gctatgccag
      361 aaacgacata acaatggaag gggacacttt ctataataaa gttcgatttt atggtaccaa
      421 ctttcccgcc aatggtccag ttatgcagaa gaagacgctg aaatgggagc cctccactga
      481 gaaaatgtat gtgcgtgatg gagtgctgac gggtgatatt catatggctt tgttgcttga
      541 aggaaatgcc cattaccgat gtgacttcag aactacttac aaagctaagg agaagggtgt
      601 caagttacca ggctaccact ttgtggacca cgccattgag attttaagcc atgacaaaga
      661 ttacaacaag gttaagctgt atgagcatgc tgttgctcat tctggattgc ctgacaatgc
      721 cagacgatcc ggactcagat ctcgagctca agcttcgaat tctagcagtt ctagagtaag
      781 cagcagagta agtggcccgc gccgccggag ccgaaagccc gaggccccga ggaggcgcag
      841 cccgagcccg accccgaccc ccggcccctc ccggcggggc ccctccttag gcgcttcctc
      901 ccatcaacac agtcggcgga gacaaggttg gctaaaggag atccgaaagc ttcagaagag
      961 cacacacctc ttgataagga agctgccctt cagccgcctg gcaagagaaa tatgtgttaa
     1021 attcactcgt gtgtgacttc aattggcagc ccaggcccta tggcctacag agcagcagag
     1081 cattctagtc atctctttga ggacgctatc tcctcactaa ctgcagcgag tactctctcc
     1141 aggaatgtgc actgacgaga tcggactgag agatggctag gtaccggatc gatcacgatc
     1201 agatacgtac tatcagctac ccattgtaag gcttaac
//
