LOCUS       sequence-103363-        1259 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, N1 Cloning Vector, Excitation:505
            / 569, Emission:516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1259)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4a-CenpB-N-22
FEATURES             Location/Qualifiers
     source          1..1259
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    458..510
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      518..527
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             524..1207
                     /codon_start=1
                     /product="fixation-resistant green-to-red photoswitchable 
                     fluorescent protein (Paez-Segala et al., 2015)"
                     /label=mEos4a
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICYA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 tccggaagag agtccgcgat cttccagaat gaagaaatcc gagctaggca gggcgaatcg
       61 cgggctccaa catccgccgt cacgctgagc acgatctgaa gaacaagcgg ccatctggcg
      121 tcgaggcgca gtacggggtg gcctccacct gcgcaagacc acagctgtct cctacgacag
      181 ctcgagggct gctcatcgcc tggtccagca gatccgcgcc gccggcctgc ccggtcaagg
      241 gcatcatcct caaggaggag gcgctgcgca tagccgagga gctgggcatg gacgacttca
      301 ccgcctccaa cggctggctg gaccgcttcc gccggcgcca cggcgtggtg tcctgcagcg
      361 gcgtggcccg cgcccgcgcg cgaaacgctg ccccccgcac cccggcggcg ctgccagtcc
      421 ggccgcggtg ccctcggagg gcagtggcgg gagcactctc gagctcaagc ttcgaattct
      481 gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtga gtgcgattaa
      541 gccagacatg aggatcaaac tccgtatgga aggcaacgta aacgggcacc actttgtgat
      601 cgacggagat ggtacaggca agccttatga gggaaaacag accatggatc ttgaagtcaa
      661 agagggcgga cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa
      721 cagggtattc gttaaatatc cagacaacat acaagactat tttaagcagt cgtttcctaa
      781 ggggtattcg tgggaacgaa gcttgacttt cgaagacggg ggcatttgct atgccagaaa
      841 cgacataaca atggaagggg acactttcta taataaagtt cgattttatg gtaccaactt
      901 tcccgccaat ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa
      961 aatgtatgtg cgtgatggag tgctgacggg tgatattcat atggctttgt tgcttgaagg
     1021 aaatgcccat taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa
     1081 gttaccaggc taccactttg tggaccacgc cattgagatt ttaagccatg acaaagatta
     1141 caacaaggtt aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag
     1201 acgataagcg gccgcgactc tagatcataa tcagccatac cacattgtag aggaatctc
//
