LOCUS       sequence-103366-        1234 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, C1 Cloning Vector, Excitation:505 / 569, 
            Emission:516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1234)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-CenpA-C-22
FEATURES             Location/Qualifiers
     source          1..1234
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..726
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 cgcatgcttg tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagtgcgatt
       61 aagccagaca tgaggatcaa actccgtatg gaaggcaacg taaacgggca ccactttgtg
      121 atcgacggag atggtacagg caagccttat gagggaaaac agaccatgga tcttgaagtc
      181 aaagagggcg gacctctgcc ttttgccttt gatatcctga ccactgcatt ccattacggc
      241 aacagggtat tcgtgaaata tccagacaac atacaagact attttaagca gtcgtttcct
      301 aaggggtatt cgtgggaacg aagcttgact ttcgaagacg ggggcatttg caatgccaga
      361 aacgacataa caatggaagg ggacactttc tataataaag ttcgatttta tggtaccaac
      421 tttcccgcca atggtccagt tatgcagaag aagacgctga aatgggagcc ctccactgag
      481 aaaatgtatg tgcgtgatgg agtgctgacg ggtgatattg agatggcttt gttgcttgaa
      541 ggaaatgccc attaccgatg tgacttcaga actacttaca aagctaagga gaagggtgtc
      601 aagttaccag gcgcccactt tgtggaccac gccattgaga ttttaagcca tgacaaagat
      661 tacaacaagg ttaagctgta tgagcatgct gttgctcatt ctggattgcc tgacaatgcc
      721 agacgatccg gactcagatc tcgagctcaa gcttcgaatt ctagcagttc tagagtaagc
      781 agcagagtaa gtggcccgcg ccgccggagc cgaaagcccg aggccccgag gaggcgcagc
      841 ccgagcccga ccccgacccc cggcccctcc cggcggggcc cctccttagg cgcttcctcc
      901 catcaacaca gtcggcggag acaaggttgg ctaaaggaga tccgaaagct tcagaagagc
      961 acacacctct tgataagaag ctgcccttca gccgcctgca gagaaatatg tgttaaattc
     1021 actcgtgtgt gacttcatgc agcccaggcc ctatgcccta cagagcagca gagcattcta
     1081 gtcatctctt tgagacgcta tctcctcact acatgcagcg agtactctct ctacgaatgt
     1141 gcactgaccg agatcggtcc tgagagacta gcctaggtac gagctggatc acggatcgat
     1201 actgatctat cagcatacca catgtgtaga gagg
//
