LOCUS       sequence-103373-        1232 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, N1 Cloning Vector, Excitation:505 / 569, 
            Emission:516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1232)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-Patched1-N-18
FEATURES             Location/Qualifiers
     source          1..1232
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      491..500
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             497..1180
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 agcgccccac ctgattcagg tcctgtcctg aacgcaaggc agacgctgag atccctagga
       61 agcttgcgca acccttacag accggcagag acgcttgatc tactgaggca tctgcctagc
      121 atagacgtca gaccccgtgg gcccgtctca cacctcgacc cacgtccacg catgggcagc
      181 tctgtgccca gctactgcag cccatcacac tgtgacggct ctgcttcggt gactgttgct
      241 gtgcatcccc cgcctggacc tgggcgcaac ccccgagggg gccctgtcca ggctatgaga
      301 gctacctgag actgatcacg gggtatttga ggatcctcat gtgccttttc atgtcaggtg
      361 tgagaggagg gactcaaagg tggaggtcat agagctacag gacgtggaat gtgaggagag
      421 gccgtggggg agcagctcca acgggagagg cagcgggagt ggcagtcgcg gaggcagtgg
      481 tggaccggtc gccaccatgg tgagtgcgat taagccagac atgaggatca aactccgtat
      541 ggaaggcaac gtaaacgggc accactttgt gatcgacgga gatggtacag gcaagcctta
      601 tgagggaaaa cagaccatgg atcttgaagt caaagagggc ggacctctgc cttttgcctt
      661 tgatatcctg accactgcat tccattacgg caacagggta ttcgtgaaat atccagacaa
      721 catacaagac tattttaagc agtcgtttcc taaggggtat tcgtgggaac gaagcttgac
      781 tttcgaagac gggggcattt gcaatgccag aaacgacata acaatggaag gggacacttt
      841 ctataataaa gttcgatttt atggtaccaa ctttcccgcc aatggtccag ttatgcagaa
      901 gaagacgctg aaatgggagc cctccactga gaaaatgtat gtgcgtgatg gagtgctgac
      961 gggtgatatt gagatggctt tgttgcttga aggaaatgcc cattaccgat gtgacttcag
     1021 aactacttac aaagctaagg agaagggtgt caagttacca ggcgcccact ttgtggacca
     1081 cgccattgag attttaagcc atgacaaaga ttacaacaag gttaagctgt atgagcatgc
     1141 tgttgctcat tctggattgc ctgacaatgc cagacgataa gcggccgcga ctctagatca
     1201 taatcagcca taccacattg tagaagaggc cc
//
