LOCUS       sequence-103377-        1215 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, N1 Cloning Vector, Excitation:505 / 569, 
            Emission:516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1215)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-Smoothened-N-18
FEATURES             Location/Qualifiers
     source          1..1215
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      475..484
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             481..1164
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 gacatatggc cccagatgtt tccgtacctt ggcatcagtc agcgagatca gcaacatgga
       61 ctggtgagca ggattcccag agtaggaagc gtttgtcgga gaaaagcgag agagaggaga
      121 gtgtgccctg agcagccctg agctcacatc tgccctgtct gccacagtgc agtctcgctg
      181 ctcagctgcc tcggcagaag tgcctggtag ctgcaaacgc ctggggaaca gggagtcctg
      241 ccgacaggga gcctggactc tagtctccaa ccccttctgc ccagagccta gtccccatca
      301 agatccattt ctccctggtg cctcagcccc ccgggtctgg gctcagggcc gcctccaggg
      361 gctgggatcc attcattccc gcactaacta atggaggctg agatcttgga tgcagactcg
      421 gacttctctc gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc
      481 atggtgagtg cgattaagcc agacatgagg atcaaactcc gtatggaagg caacgtaaac
      541 gggcaccact ttgtgatcga cggagatggt acaggcaagc cttatgaggg aaaacagacc
      601 atggatcttg aagtcaaaga gggcggacct ctgccttttg cctttgatat cctgaccact
      661 gcattccatt acggcaacag ggtattcgtg aaatatccag acaacataca agactatttt
      721 aagcagtcgt ttcctaaggg gtattcgtgg gaacgaagct tgactttcga agacgggggc
      781 atttgcaatg ccagaaacga cataacaatg gaaggggaca ctttctataa taaagttcga
      841 ttttatggta ccaactttcc cgccaatggt ccagttatgc agaagaagac gctgaaatgg
      901 gagccctcca ctgagaaaat gtatgtgcgt gatggagtgc tgacgggtga tattgagatg
      961 gctttgttgc ttgaaggaaa tgcccattac cgatgtgact tcagaactac ttacaaagct
     1021 aaggagaagg gtgtcaagtt accaggcgcc cactttgtgg accacgccat tgagatttta
     1081 agccatgaca aagattacaa caaggttaag ctgtatgagc atgctgttgc tcattctgga
     1141 ttgcctgaca atgccagacg ataagcggcc gcgactctag atcataatca gccataccac
     1201 attgtagaga tgcac
//
