LOCUS       sequence-103384-        1384 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Bundling , C1 Cloning Vector, Excitation:399, 
            Emission:456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1384)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagBFP2-Espin-Like-C-18
FEATURES             Location/Qualifiers
     source          1..1384
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             42..752
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 cccctgtcta gttgacgtca gatccgctag cggtggccac catggtgtct aagggcgaag
       61 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc
      121 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
      181 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc
      241 tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt
      301 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
      361 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
      421 gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
      481 tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga
      541 agctcgtggg cgggagccat ctgatcgcaa acgccaagac cacatataga tccaagaaac
      601 ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca
      661 aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg
      721 acctccctag caaactgggg cacaagctta attccggact cagatctcga gctcaagctt
      781 cgaattccgg tggcagcggc gggagtgagg cgcagcaggc actggtggcc tccaaggatg
      841 gggatatggc caccttggaa cggctatttg aggctggtgc cctaagacca gacatcactg
      901 atgatctggg ggctggcctg tgcatcatgc cacccgggct ggtcacctgg actgtgtcaa
      961 gttcctggta cagagagcca agctgccagg caccagcagg cacacaacgg ggcacaccag
     1021 tgcacgatgc agcagccacg ggcaacctgg cagagctgtg ctggctggtc cgtgatgcgg
     1081 ctgcggctgc aggaccaaga tgcctcaggt ttcccctccg ctgcacctgc tgcccggttt
     1141 ggacacccag ccctggtggg agtggttact ctctggaggc catgccagcc cacactggga
     1201 gagcgctgga gggcttaaca ctgcacccac cgtgcctgta attggtgatc tgacctgtct
     1261 gaatccttga cacgcggccc ccagacagtg tgtggtacac gactgtcagt gggtcctctc
     1321 acctctatct gtgtctgtcg aagagcccac tgctctactg tgcatcaatc tccggtgtag
     1381 tgga
//
