LOCUS       sequence-103389-        1311 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, N1 Cloning Vector, Excitation:399, 
            Emission:456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1311)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagBFP2-TES-N-14
FEATURES             Location/Qualifiers
     source          1..1311
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      539..548
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             545..1255
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 tcttaatgat agaaagagag gtacacgcca tttatggcga aagctgctag atagcttgac
       61 cagctgtttg tgcgacgaca ctgccatgac ctgtgacatg atttatttgg aagaataaga
      121 gctatactgg cagacatact tgacagcgag aaacccgatg tgctgctgga cgagctgata
      181 tcagcatgag tatacccagc agaaacagat gcactgaaac actctgctgc ttgactgtga
      241 tagcatctag ctgggagata tacgtgatgt caatgacagc ccgtgtgcag cctgctatgt
      301 gaagaatcac gctgtggtgt gtcaaggatg ccacaatgcc atcgacccag aagtgcagcg
      361 ggtgacctat aacaatttca gctggcatgc atccacagag tgctttctgt gctcttgctg
      421 cagcaaatgc ctcattgggc agaagttcat gccagtagaa gggatggttt tctgttcagt
      481 ggaatgtaag aagaggatgt cttccggcgg tagcggcggt agcggggatc caccggtcgc
      541 caccatggtg tctaagggcg aagagctgat taaggagaac atgcacatga agctgtacat
      601 ggagggcacc gtggacaacc atcacttcaa gtgcacatcc gagggcgaag gcaagcccta
      661 cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt
      721 cgacatcctg gctactagct tcctctacgg cagcaagacc ttcatcaacc acacccaggg
      781 catccccgac ttcttcaagc agtccttccc tgagggcttc acatgggaga gagtcaccac
      841 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct
      901 catctacaac gtcaagatca gaggggtgaa cttcacatcc aacggccctg tgatgcagaa
      961 gaaaacactc ggctgggagg ccttcaccga gacgctgtac cccgctgacg gcggcctgga
     1021 aggcagaaac gacatggccc tgaagctcgt gggcgggagc catctgatcg caaacgccaa
     1081 gaccacatat agatccaaga aacccgctaa gaacctcaag atgcctggcg tctactatgt
     1141 ggactacaga ctggaaagaa tcaaggaggc caacaacgag acctacgtcg agcagcacga
     1201 ggtggcagtg gccagatact gcgacctccc tagcaaactg gggcacaagc ttaattaagc
     1261 ggccgcgact ctagatcata atcagccata ccacattgta gaggtatcct c
//
