LOCUS       sequence-10339-e        7619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7619)
  TITLE     Yeast ADE1 plasmids for classroom transformation
REFERENCE   2  (bases 1 to 7619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: YEpADE1
FEATURES             Location/Qualifiers
     source          1..7619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2168..2185)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2168..2184)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2177..2199)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2621..3967
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        complement(5209..5607)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(5627..5645)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5713..5817
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5818..5886,5887..6678)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6036..6055)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6849..7437
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7338..7357
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7591..7608
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttat
      241 tcacgagtca gtctgactct tgcgagagat gaggatgtaa taatactaat ctcgaagatg
      301 ccatctaata catatagaca tacatatata tatatataca ttctatatat tcttacccag
      361 attctttgag gtaagacggt tgggttttat cttttgcagt tggtactatt aagaacaatc
      421 gaatcataag cattgcttac aaagaataca catacgaaat attaacgata atgtcaatta
      481 cgaagactga actggacggt atattgccat tggtggccag aggtaaagtt agagacatat
      541 atgaggtaga cgctggtacg ttgctgtttg ttgctacgga tcgtatctct gcatatgacg
      601 ttattatgga aaacagcatt cctgaaaagg ggatcctatt gaccaaactg tcagagttct
      661 ggttcaagtt cctgtccaac gatgttcgta atcatttggt cgacatcgcc ccaggtaaga
      721 ctattttcga ttatctacct gcaaaattga gcgaaccaaa gtacaaaacg caactagaag
      781 accgctctct attggttcac aaacataaac taattccatt ggaagtaatt gtcagaggct
      841 acatcaccgg atctgcttgg aaagagtacg taaaaacagg tactgtgcat ggtttgaaac
      901 aacctcaagg acttaaagaa tctcaagagt tcccagaacc aatcttcacc ccatcgacca
      961 aggctgaaca aggtgaacat gacgaaaaca tctctcctgc ccaggccgct gagctggtgg
     1021 gtgaagattt gtcacgtaga gtggcagaac tggctgtaaa actgtactcc aagtgcaaag
     1081 attatgctaa ggagaagggc atcatcatcg cagacactaa attcgaattc ggtattgacg
     1141 aaaagaccaa tgaaattatt ctagtggacg aggtgctaac gccagactcc tctagattct
     1201 ggaacggtgc ctcttataag gtaggagaat cccaagattc ttacgataag caatttttaa
     1261 gagactggct tactgctaat aagttgaacg gtgttaacgg cgtcaaaatg ccccaagaca
     1321 ttgtcgacag gacaagggcc aaatatatag aggcttatga aacattgaca gggtctaaat
     1381 ggtctcacta acgtgattta catatactac aagtcgccag tgtaactcct cactgaatat
     1441 gattcataca tacccgtatg tattaatgta taaatgttct cagagcaaat tttatcgata
     1501 tcttgtttgc cagtggtatg caggtttggc aaatttttta ccataatatc cgtttataga
     1561 ttctggaacc ttaccaactt tcttaccgct aattacttcc ctggctcgct cctccactgc
     1621 ctgggtaaat tgttccttca actgactcag ttctctttcg tattcaatag cttgcttctc
     1681 gaggattttt tcaatgtttg tcagctcatt ttcatagtcc agtaacttcc tttcaaatct
     1741 ctctaattgc aacgactttc ttgcagttcg tatctgaata tcttgcagta attcaaaagt
     1801 ggaaggcctg gttcttaagt tcacatctat cattgaatgt attatggcat taagccctct
     1861 agagtaatac tcagggacgg tgtcacattt cccgttttta atcttagttt gtagctcgag
     1921 ataatttttt gcctgaaatg gggggtgcaa cgaacacatc tcaaaaataa cacaacctag
     1981 tgaccagatg tcggatagtg gggagtatgg ttggtccatc aacacttcag gcgacatgta
     2041 atatggtgta ccgacgtatg ttgtggcaaa ttgaatacta gtttccagag atttggctaa
     2101 cccaaaatca cctaacttta ccacaacttg actatagtcc atagggctcc cccttttccc
     2161 tgaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     2221 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     2281 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
     2341 ttctccttac gcatctgtgc ggtatttcac accgcatata tcggatcgta cttgttaccc
     2401 atcattgaat tttgaacatc cgaacctggg agttttccct gaaacagata gtatatttga
     2461 acctgtataa taatatatag tctagcgctt tacggaagac aatgtatgta tttcggttcc
     2521 tggagaaact attgcatcta ttgcataggt aatcttgcac gtcgcatccc cggttcattt
     2581 tctgcgtttc catcttgcac ttcaatagca tatctttgtt aacgaagcat ctgtgcttca
     2641 ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg
     2701 catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc
     2761 ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg
     2821 agctgcattt ttacagaaca gaaatgcaac gcgagagcgc tattttacca acaaagaatc
     2881 tatacttctt ttttgttcta caaaaatgca tcccgagagc gctatttttc taacaaagca
     2941 tcttagatta ctttttttct cctttgtgcg ctctataatg cagtctcttg ataacttttt
     3001 gcactgtagg tccgttaagg ttagaagaag gctactttgg tgtctatttt ctcttccata
     3061 aaaaaagcct gactccactt cccgcgttta ctgattacta gcgaagctgc gggtgcattt
     3121 tttcaagata aaggcatccc cgattatatt ctataccgat gtggattgcg catactttgt
     3181 gaacagaaag tgatagcgtt gatgattctt cattggtcag aaaattatga acggtttctt
     3241 ctattttgtc tctatatact acgtatagga aatgtttaca ttttcgtatt gttttcgatt
     3301 cactctatga atagttctta ctacaatttt tttgtctaaa gagtaatact agagataaac
     3361 ataaaaaatg tagaggtcga gtttagatgc aagttcaagg agcgaaaggt ggatgggtag
     3421 gttatatagg gatatagcac agagatatat agcaaagaga tacttttgag caatgtttgt
     3481 ggaagcggta ttcgcaatat tttagtagct cgttacagtc cggtgcgttt ttggtttttt
     3541 gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc tctgaagttc ctatactttc
     3601 tagctagaga ataggaactt cggaatagga acttcaaagc gtttccgaaa acgagcgctt
     3661 ccgaaaatgc aacgcgagct gcgcacatac agctcactgt tcacgtcgca cctatatctg
     3721 cgtgttgcct gtatatatat atacatgaga agaacggcat agtgcgtgtt tatgcttaaa
     3781 tgcgtactta tatgcgtcta tttatgtagg atgaaaggta gtctagtacc tcctgtgata
     3841 ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc actacccttt agctgttcta
     3901 tatgctgcca ctcctcaatt ggattagtct catccttcaa tgctatcatt tcctttgata
     3961 ttggatcgat ccgatgataa gctgtcaaac atgagaatta attctaccct atgaacatat
     4021 tccattttgt aatttcgtgt cgtttctatt atgaatttca tttataaagt ttatgtacaa
     4081 atatcataaa aaaagagaat ctttttaagc aaggattttc ttaacttctt cggcgacagc
     4141 atcaccgact tcggtggtac tgttggaacc acctaaatca ccagttctga tacctgcatc
     4201 caaaaccttt ttaactgcat cttcaatggc cttaccttct tcaggcaagt tcaatgacaa
     4261 tttcaacatc attgcagcag acaagatagt ggcgataggg tcaaccttat tctttggcaa
     4321 atctggagca gaaccgtggc atggttcgta caaaccaaat gcggtgttct tgtctggcaa
     4381 agaggccaag gacgcagatg gcaacaaacc caaggaacct gggataacgg aggcttcatc
     4441 ggagatgata tcaccaaaca tgttgctggt gattataata ccatttaggt gggttgggtt
     4501 cttaactagg atcatggcgg cagaatcaat caattgatgt tgaaccttca atgtaggaaa
     4561 ttcgttcttg atggtttcct ccacagtttt tctccataat cttgaagagg ccaaaacatt
     4621 agctttatcc aaggaccaaa taggcaatgg tggctcatgt tgtagggcca tgaaagcggc
     4681 cattcttgtg attctttgca cttctggaac ggtgtattgt tcactatccc aagcgacacc
     4741 atcaccatcg tcttcctttc tcttaccaaa gtaaatacct cccactaatt ctctgacaac
     4801 aacgaagtca gtacctttag caaattgtgg cttgattgga gataagtcta aaagagagtc
     4861 ggatgcaaag ttacatggtc ttaagttggc gtacaattga agttctttac ggatttttag
     4921 taaaccttgt tcaggtctaa cactacctgt accccattta ggaccaccca cagcacctaa
     4981 caaaacggca tcaaccttct tggaggcttc cagcgcctca tctggaagtg ggacacctgt
     5041 agcatcgata gcagcaccac caattaaatg attttcgaaa tcgaacttga cattggaacg
     5101 aacatcagaa atagctttaa gaaccttaat ggcttcggct gtgatcttga ccaacgtggt
     5161 cacctggcaa aacgacgatc ttcttagggg cagacatagg ggcagacatt agaatgtata
     5221 tccttgaaat atatatatat attgctgaaa tgtaaaaggt aagaaaagtt agaataagac
     5281 gattgctaac cacctattgg aaaaaacaat aggtccttaa ataatattgt caacttcaag
     5341 tattgtgatg caagcattta gtcatgaacg cttctctatt ctatatgaaa agccggttcc
     5401 ggcctctcac ctttcctttt tctcccaatt tttcagttga aaaaggtata tgcgcggcga
     5461 cctctgaaat taacaaaaaa tttccagtca tcgaatttgt ctgtgcgata gcgcccctgt
     5521 gtgttctcgt tatgttgagg aaaaaaataa tggttgctaa gagattcgaa ctcttgcatc
     5581 ttacgatacc tgagtattcc cacagttaat tcttgaagac gaaagggcct cgtgatacgc
     5641 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     5701 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5761 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5821 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5881 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5941 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     6001 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     6061 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     6121 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     6181 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     6241 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     6301 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     6361 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6421 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6481 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     6541 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6601 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6661 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6721 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6781 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6841 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6901 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6961 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     7021 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7081 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7141 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7201 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7261 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7321 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7381 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7441 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7501 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7561 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaaga
//
