LOCUS       sequence-103394-        1284 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubule-Binding Protein, C1 Cloning Vector, 
            Excitation:515, Emission:528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1284)
  TITLE     Michael Davidson Unpublished
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-RatLC3-C-7
FEATURES             Location/Qualifiers
     source          1..1284
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(46..48,49..51,52..762)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(91..112)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(352..371)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     699..720
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggcatgctag tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
       61 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      121 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      181 aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      241 ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
      301 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      361 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      421 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      481 tacaacagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
      541 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag
      601 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
      661 tccaaactga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
      721 accgccgccg ggatcactct cggcatggac gagctgtaca agtccggact cagatcctct
      781 agaccgtccg agaagacctt caaacagcgc cggagcttcg aacaaagagt ggaagatgtc
      841 cggctcatcc gggagcagca ccccaccaag atcccagtga ttatagagcg atacaagggt
      901 gagaagcagc tgcccgtcct ggacaagacc aagttccttg tacctgatca cgtgaatatg
      961 agcgaactca tcaagataat tagaaggcgc ctgcagctca atgctaacca agccttcttc
     1021 ctcctggtga atgggcacag catggtgagt gtgtccacac ccatcttctg agtgtacgag
     1081 agcgagagag atgagacggc ttcctgtaca tggatctatg cctcccagga gaacgttcgg
     1141 acagcactgc tgtacataca tgttcagctc tctgagcaca gcacaggaaa gaggagcaat
     1201 gcttggacga acatacagtc cacctcaact aaagacaagc cctctgggtc tccagttgct
     1261 aacactggtg agagtgatag aaga
//
