LOCUS       sequence-10341-e        8974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Meylan E, Dooley AL, Feldser DM, Shen L, Turk E, Ouyang C, Jacks T
  TITLE     Requirement for NF-kappaB signalling in a mouse model of lung 
            adenocarcinoma.
  JOURNAL   Nature. 2009 Nov 5;462(7269):104-7. doi: 10.1038/nature08462. Epub 
            2009 Oct 21.
  PUBMED    19847165
REFERENCE   2  (bases 1 to 8974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature08462"; journalName: "Nature"; date: "2009-11-5- 5"; 
            volume: "462"; issue: "7269"; pages: "104-7"
COMMENT     Sequence Label: TRE.IkBSR-Flag Pgk.Cre
FEATURES             Location/Qualifiers
     source          1..8974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1485..1508
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        1553..2052
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1588..1611)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1985..2004
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2077..2097
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2098..3126
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     primer_bind     complement(3152..3172)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3570..3581)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3786..4019
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4091..4212
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4128..4147)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4182..4201
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4252..4387
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4407..4426)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4408..4426)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4436..4453)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4436..4452)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4445..4467)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4594..5049
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4681..4700)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4891..4912
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5075..5179
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5180..5248,5249..6040)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5398..5417)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6211..6799
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6700..6719
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6953..6970
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7087..7108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7123..7128,7129..7146,7147..7153)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7161..7177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7166..7188
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7185..7201
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7185..7201
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7220..7240
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7222..7240
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7268..7494
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7495..7675
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7722..7847
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8340..8573
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8758..8802
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattcgcca
      541 ccatgtttca gccagctggg cacggccagg actgggccat ggagggcccg cgggatggcc
      601 tcaagaagga gcgcttggtg gacgatcgcc acgacgccgg cctggacgcc atgaaggacg
      661 aggagtacga gcaaatggtg aaggagctgc gggagatccg cctgcagccg caggaggcgc
      721 cgctggccgc cgagccctgg aagcagcagc tcacggagga cggagactcg ttcctgcact
      781 tggcaatcat ccacgaagag aagccgctga ccatggaagt cattggtcag gtgaagggag
      841 acctggcctt cctcaacttc cagaacaacc tgcagcagac tccactccac ttggctgtga
      901 tcaccaacca gccaggaatt gctgaggcac ttctgaaagc tggctgtgat cctgagctcc
      961 gagactttcg aggaaatacc cctctacatc ttgcctgtga gcagggctgc ctggccagtg
     1021 tagcagtctt gacgcagacc tgcacacccc agcatctcca ctccgtcctg caggccacca
     1081 actacaatgg ccacacgtgt ctgcacctag cctctatcca cggctacctg gccatcgtgg
     1141 agcacttggt gactttgggt gctgatgtca acgctcagga gccctgcaat ggccggacag
     1201 ccctccacct tgcggtggac ctgcagaatc ctgacctggt ttcgctcttg ttgaaatgtg
     1261 gggctgatgt caacagggta acctaccaag gctactcccc ctaccagctt acctggggcc
     1321 gcccaagtac ccggatacag cagcagctgg gccagctgac cctggaaaat ctccagatgc
     1381 tacccgagag cgaggatgag gagagctatg acacggagtc agaattaaca gaggatgagc
     1441 tgccctatga tgactgtgtg tttggaggcc agcgtctgac attagattac aaagacgatg
     1501 acgataaata agaattcacg cgtatgcatg tttaaaccct cgaaattcta ccgggtaggg
     1561 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     1621 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
     1681 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     1741 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     1801 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     1861 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     1921 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
     1981 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
     2041 ccgggccttt cgacctgcat ccatgctagc accatgccca agaagaagag gaaggtgtcc
     2101 aatttactga ccgtacacca aaatttgcct gcattaccgg tcgatgcaac gagtgatgag
     2161 gttcgcaaga acctgatgga catgttcagg gatcgccagg cgttttctga gcatacctgg
     2221 aaaatgcttc tgtccgtttg ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa
     2281 tggtttcccg cagaacctga agatgttcgc gattatcttc tatatcttca ggcgcgcggt
     2341 ctggcagtaa aaactatcca gcaacatttg ggccagctaa acatgcttca tcgtcggtcc
     2401 gggctgccac gaccaagtga cagcaatgct gtttcactgg ttatgcggcg gatccgaaaa
     2461 gaaaacgttg atgccggtga acgtgcaaaa caggctctag cgttcgaacg cactgatttc
     2521 gaccaggttc gttcactcat ggaaaatagc gatcgctgcc aggatatacg taatctggca
     2581 tttctgggga ttgcttataa caccctgtta cgtatagccg aaattgccag gatcagggtt
     2641 aaagatatct cacgtactga cggtgggaga atgttaatcc atattggcag aacgaaaacg
     2701 ctggttagca ccgcaggtgt agagaaggca cttagcctgg gggtaactaa actggtcgag
     2761 cgatggattt ccgtctctgg tgtagctgat gatccgaata actacctgtt ttgccgggtc
     2821 agaaaaaatg gtgttgccgc gccatctgcc accagccagc tatcaactcg cgccctggaa
     2881 gggatttttg aagcaactca tcgattgatt tacggcgcta aggatgactc tggtcagaga
     2941 tacctggcct ggtctggaca cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct
     3001 ggagtttcaa taccggagat catgcaagct ggtggctgga ccaatgtaaa tattgtcatg
     3061 aactatatcc gtaacctgga tagtgaaaca ggggcaatgg tgcgcctgct ggaagatggc
     3121 gattagtggc cagatatcgt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3181 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3241 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     3301 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     3361 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     3421 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     3481 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     3541 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     3601 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     3661 ggatctccct ttgggccgcc tccccgcctg tttcgcctcg gcgtccggtc tagaggtacc
     3721 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     3781 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     3841 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     3901 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     3961 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4021 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     4081 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4141 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4201 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     4261 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     4321 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     4381 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     4441 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     4501 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     4561 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     4621 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4681 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4741 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4801 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4861 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4921 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     4981 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     5041 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5101 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5161 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5221 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5281 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5341 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5401 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5461 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5521 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5581 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5641 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5701 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5761 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5821 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5881 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5941 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6001 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6061 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6121 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6181 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6241 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6301 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6361 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6421 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6481 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6541 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6601 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6661 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6721 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6781 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6841 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6901 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6961 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     7021 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     7081 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     7141 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     7201 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
     7261 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
     7321 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
     7381 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
     7441 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
     7501 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7561 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7621 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     7681 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     7741 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     7801 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     7861 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     7921 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     7981 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     8041 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     8101 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     8161 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     8221 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     8281 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     8341 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     8401 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     8461 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     8521 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     8581 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     8641 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     8701 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     8761 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     8821 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     8881 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     8941 tcgtttcaga cccacctccc aaccccgagg ggac
//
