LOCUS       sequence-103414-        1071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Fujii H, Fujita T
  TITLE     An enChIP system for the analysis of genome functions in budding 
            yeast.
  JOURNAL   Biol Methods Protoc. 2022 Oct 17;7(1):bpac025. doi: 
            10.1093/biomethods/bpac025. eCollection 2022.
  PUBMED    36325175
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Methods Protoc."; date: "2022-10-17"; volume: "7(1):bpac025. doi"; 
            pages: " 10.1093/biomethods/bpac025. eCollection 2022"
COMMENT     Sequence Label: 3xFLAG-dCas9/pTEF1p-CYC1t
FEATURES             Location/Qualifiers
     source          1..1071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..59
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaagc
       61 tcatggacaa gaagtactcc attgggctcg ctatcggcac aaacagcgtc ggctgggccg
      121 tcattacgga cgagtacaag gtgccgagca aaaaattcaa agttctgggc aataccgatc
      181 gccacagcat aaagaagaac ctcattggcg ccctcctgtt cgactccggg gagacggccg
      241 aagccacgcg gctcaaaaga acagcacggc gcagatatac ccgcagaaag aatcggatct
      301 gctacctgca ggagatcttt agtaatgaga tggctaaggt ggatgactct ttcttccata
      361 ggctggagga gtcctttttg gtggaggagg ataaaaagca cgagcgccac ccaatctttg
      421 gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat catctgagga
      481 agaagcttgt agacagtact gataaggctg acttgcggtt gatctatctc gcgctggcgc
      541 atatgatcaa atttcgggga cacttcctca tcgaggggga cctgaaccca gacaacagcg
      601 atgtcgacaa actctttatc caactggttc agacttacaa tcagcttttc gaagagaacc
      661 cgatcaacgc atccggagtt gacgccaaag caatcctgag cgctaggctg tccaaatccc
      721 ggcggctcga aaacctcatc gcacagctcc ctggggagaa gaagaacggc ctgtttggta
      781 atcttatcgc cctgtcactc gggctgaccc ccaactttaa atctaacttc gacctggccg
      841 aagatgccaa gcttcaactg agcaaagaca cctacgatga tgatctcgac aatctgctgg
      901 cccagatcgg cgaccagtac gcagaccttt ttttggcggc aaagaacctg tcagacgcca
      961 ttctgctgag tgatattctg cgagtgaaca cggagatcac caaagctccg ctgagcgcta
     1021 gtatgatcaa gcgctatgat gagcaccacc aagacttgac tttgctgaag g
//
