LOCUS       sequence-103418-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IPTG-inducible SCRIBE_galK(OFF).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Farzadfard F, Lu TK
  TITLE     Synthetic biology. Genomically encoded analog memory with precise in
            vivo DNA writing in living cell populations.
  JOURNAL   Science. 2014 Nov 14;346(6211):1256272. doi: 
            10.1126/science.1256272.
  PUBMED    25395541
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2014-11-14"; volume: "346(6211):1256272. doi"; pages: " 
            10.1126/science.1256272"
COMMENT     Sequence Label: pFF714
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(48..833)
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
ORIGIN
        1 gtctttcgac tgagcctttc gttttatttg atgcctctag cacgcgttca tgctgccacc
       61 ttctgctctg cggctttctg tttcaggaat ccaagagctt ttactgcttc ggcctgtgtc
      121 agttctgacg atgcacgaat gtcgcggcga aatatctggg aacagagcgg caataagtcg
      181 tcatcccatg ttttatccag ggcgatcagc agagtgttaa tctcctgcat ggtttcatcg
      241 ttaaccggag tgatgtcgcg ttccggctga cgttctgcag tgtatgcagt attttcgaca
      301 atgcgctcgg cttcatcctt gtcatagata ccagcaaatc cgaaggccag acgggcacac
      361 tgaatcatgg ctttatgacg taacatccgt ttgggatgcg actgccacgg ccccgtgatt
      421 tctctgcctt cgcgagtttt gaatggttcg cggcggcatt catccatcca ttcggtaacg
      481 cagatcggat gattacggtc cttgcggtaa atccggcatg tacaggattc attgtcctgc
      541 tcaaagtcca tgccatcaaa ctgctggttt tcattgatga tgcgggacca gccatcaacg
      601 cccaccaccg gaacgatgcc attctgctta tcaggaaagg cgtaaatttc tttcgtccac
      661 ggattaaggc cgtactggtt ggcaacgatc agtaatgcga tgaactgcgc atcgctggca
      721 tcacctttaa atgccgtctg gcgaagagtg gtgatcagtt cctgtgggtc gacagaatcc
      781 atgccgacac gttcagccag cttcccagcc agcgttgcga gtgcagtact cattcgtttt
      841 atacctctga at
//
