LOCUS       sequence-103421-         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Fujii H, Fujita T
  TITLE     An enChIP system for the analysis of genome functions in budding 
            yeast.
  JOURNAL   Biol Methods Protoc. 2022 Oct 17;7(1):bpac025. doi: 
            10.1093/biomethods/bpac025. eCollection 2022.
  PUBMED    36325175
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Methods Protoc."; date: "2022-10-17"; volume: "7(1):bpac025. doi"; 
            pages: " 10.1093/biomethods/bpac025. eCollection 2022"
COMMENT     Sequence Label: 3xFLAG-dCas9/pTEF1p-CYC1t
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..77
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      93..340
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(109..127)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(358..377)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(359..377)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(387..404)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(387..403)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(396..418)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(631..650)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggctctatga aacaagaatc gacctctctc agctcggtgg agacagcagg gctgacccca
       61 agaagaagag gaaggtgtga tctcttctcg agtcatgtaa ttagttatgt cacgcttaca
      121 ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt
      181 ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat ttatatttca
      241 aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt
      301 gcttgagaag gttttgggac gctcgaaggc tttaatttgc ggccggtacc caattcgccc
      361 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
      421 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      481 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      541 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      601 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      661 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      721 tta
//
