LOCUS       sequence-10343-e        4011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Zeiser E, Frokjaer-Jensen C, Jorgensen E, Ahringer J
  TITLE     MosSCI and gateway compatible plasmid toolkit for constitutive and 
            inducible expression of transgenes in the C. elegans germline.
  JOURNAL   PLoS One. 2011;6(5):e20082. Epub 2011 May 26.
  PUBMED    21637852
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(5)"; pages: "e20082. Epub 2011 May 26"
COMMENT     Sequence Label: pJA281
FEATURES             Location/Qualifiers
     source          1..4011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    2057..2180
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2252..2271)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2253..2271)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2276..2292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2276..2292)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2394..2413)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2405..3214
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2472..2491)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3361..3949
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3850..3869
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgaa atatcagttt ttaaaaaatt aaaccataaa
      721 acaaataata taacccaatt tttacatcaa accacaagaa aaaaatacat ttgggcccac
      781 ggataaagaa attaaaaaaa tacatttttt aaaggcgcac cgaattaaaa ttcatttggg
      841 tcttaccgcg tataccgtac tccgtttgtt tgatcatttt tgtcagcgct ggcggttgtt
      901 ttttcatttc atttctgctt caaagacgtt ttctcgaata atttttcgtt tattctcttt
      961 tttaaaatta atttctagcc gtaaatgtta taaattcacc catttaacgc aaatttcatg
     1021 gtaatctcat ggaaaaatgc agtttctttg ttaaagaaag cttaaatagc aaaaattccc
     1081 cgactttccc caaaatcctg ctcgattttc cgttttctca ttgtattctc tcttaattaa
     1141 ttttatcgat aatcaattga atgtttcaga cagagaatgg tctcaaaggg tgaagaagat
     1201 aacatggcaa ttattaaaga gtttatgcgt ttcaaggtgc atatggaggg atctgtcaat
     1261 gggcatgagt ttgaaattga aggtgaagga gaaggccgac catatgaggg aacacaaacc
     1321 gcaaaactaa aggtaagttt aaacatatat atactaacta accctgatta tttaaatttt
     1381 caggtaacta aaggcggacc attaccattc gcctgggaca tcctctctcc acagttcatg
     1441 tatggaagta aagcttatgt taaacatccg gcagatatac cagattattt gaaactttca
     1501 ttcccggagg gttttaagtg ggaacgcgta atgaattttg aagacggagg agttgttaca
     1561 gtgacgcaag actcaaggta agtttaaaca gttcggtact aactaaccat acatatttaa
     1621 attttcagcc tccaagatgg agaatttatt tataaagtca aacttcgagg aacgaatttc
     1681 ccctcggatg gacctgttat gcagaagaag actatgggat gggaagcttc aagtgaaaga
     1741 atgtaccctg aagacggtgc tcttaaggga gagattaaac aacgtcttaa attgaaagat
     1801 ggaggacatt acgatgctga ggtaagttta aacatgattt tactaactaa ctaatctgat
     1861 ttaaattttc aggtgaagac aacttacaaa gccaaaaaac cagttcagct gccaggagcg
     1921 tacaatgtta atattaaact ggatatcacc tcccacaacg aggattacac tatcgttgag
     1981 caatatgaaa gagctgaagg gcggcactcg acaggtggca tggatgaatt gtataaggga
     2041 ggtggaggtg gagcttcaag tttgtacaaa aaagttgaac gagaaacgta aaatgatata
     2101 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
     2161 caacatatgc agtcactatg aatcaactac ttagatggta ttagtgacct gtagaattcg
     2221 agctctagag ctgcagggcg gccgcgatat cccctatagt gagtcgtatt acatggtcat
     2281 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     2341 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     2401 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc
     2461 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     2521 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     2581 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     2641 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     2701 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     2761 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     2821 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     2881 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     2941 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     3001 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     3061 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     3121 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     3181 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca
     3241 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa
     3301 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3361 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3421 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3481 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     3541 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3601 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3661 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3721 ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg
     3781 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3841 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3901 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3961 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t
//
