LOCUS       sequence-103438-         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Destination vector for use 
            with pMuLE Entry vectors. Co-expresses firefly luciferase under the 
            control of a PGK promoter. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE Lenti Dest Luc2
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    62..186
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(211..216,217..234,235..241)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(361..380)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ttttatcgtc cagtgtggtg gaattgagta ttccagtgtg ggtggaattc tgcagatatc
       61 aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
      121 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
      181 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat
      241 gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag
      301 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
      361 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
      421 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
      481 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg
      541 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
      601 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
      661 gatgtggcgt gttacggtg
//
