LOCUS       sequence-103441-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Borcherds W, Theillet FX, Katzer A, Finzel A, Mishall KM, Powell AT,
            Wu H, Manieri W, Dieterich C, Selenko P, Loewer A, Daughdrill GW
  TITLE     Disorder and residual helicity alter p53-Mdm2 binding affinity and 
            signaling in cells.
  JOURNAL   Nat Chem Biol. 2014 Dec;10(12):1000-2. doi: 10.1038/nchembio.1668. 
            Epub 2014 Nov 2.
  PUBMED    25362358
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1668"; journalName: "Nat Chem Biol"; date: 
            "2014-12"; volume: "10"; issue: "12"; pages: "1000-2"
COMMENT     Sequence Label: pET28A-p53 (1-73) (P12A P13A)
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(284..301)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(311..328)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             347..369
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    384..408
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(408..427)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(409..427)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(511..530)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tctttcgggc ttgtagcagc cggatctcag tggtggtggt ggtggtgctc gagtcacacg
       61 cggggtagca gcctctggca ttctgggagc ttcatctgga cctgggtctt cagatgaacc
      121 attgttcaat atcgtccggg gacagcatca aatcatccat tgcttgggac ggcaaggggg
      181 acagaacgtt gttttcagga agtagtttcc ataggtctga aaatgtttcc tgactcagag
      241 cggcctcgac gctaggatct gactgcggct cctccatcat atggctgccg cgcggcacca
      301 ggccgctgct gtgatgatga tgatgatggc tgctgcccat ggtatatctc cttcttaaag
      361 ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt
      421 cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc gtggccggca
      481 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
      541 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
      601 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
      661 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
      721 agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag
      781 cgcccggaag aga
//
