LOCUS       sequence-103442-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Destination vector for use 
            with pMuLE Entry vectors. Co-expresses firefly luciferase under the 
            control of a PGK promoter. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE Lenti Dest Luc2
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    45..169
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(210..515)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(312..331)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tccagaggtt gattgtcgag cggccgccac tgtgctggat atcaaccact ttgtacaaga
       61 aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
      121 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg tcgacctgca
      181 gactggctgt gtataaggga gcctgacatt tatattcccc agaacatcag gttaatggcg
      241 tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat aacggagacc
      301 ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc
      361 gggtaaagtt cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc
      421 atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg
      481 ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt tctcgtcagc
      541 aaaagagccg ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc
      601 tttccagcgt tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc
      661 ggagatattg acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact
      721 gtaatacgct gcttcatagc atacctc
//
