LOCUS       sequence-103446-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Destination vector for use 
            with pMuLE Entry vectors. Co-expresses Neomycin cassette under the 
            control of a PGK promoter. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE Lenti Dest Neo
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             70..303
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(536..555)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg
       61 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
      121 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
      181 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
      241 agagagaccc agtacaagca aaaagcagat cttgtcttcg ttgggagtga gctagccctt
      301 ccagtccccc cttttctttt aaaaagtggc taagatctac agctgccttg taagtcattg
      361 gtcttaaagg taccgggccc tcgagatgca tacgcgtcgg tcatttcgaa ccccagagtc
      421 ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
      481 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
      541 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
      601 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
      661 tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg
      721 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gac
//
