LOCUS       sequence-103448-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Destination vector for use 
            with pMuLE Entry vectors. Co-expresses IFP1.4 under the control of a
            PGK promoter. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE Lenti Dest IFP1.4
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      58..67
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atctccgggc ctttcgacct gcagcccaag cttaccacac tcctgcatct taagcttgcc
       61 accatggctc gggaccctct gccattcttt ccacctctgt acctgggcgg ccctgagatt
      121 acaaccgaga actgcgagag agagcctatc cacattcctg ggtccatcca gccacacggg
      181 gctctgctca cagctgacgg ccactccgga gaggtgctcc aagtgtccct gaatgccgct
      241 accttcctgg gccaggagcc tactgtgctg cgggggcaga ccctggctgc cctgctcccc
      301 gagcagtggc cagccctgca ggcagccctg cccccaggat gtccagatgc cctccaatac
      361 agggccaccc tcgactggcc agctgctggg cacctcagcc tgactgtgca tcgggtggct
      421 gaactcctga tcctggagtt cgaacctacc gaggcctggg acagcattgg ccctcacgcc
      481 ctgaggaacg ccatgtttgc tctggaaagc gctccaaacc tgcgggctct ggccgaagtc
      541 gcaacacaaa ctgtgagaga actgactggc ttcgatcggg tgatgctgta caaatttgcc
      601 cctgacgcca ctggagagat gattgctgag gccagacggg agggcatgca ggcttttctg
      661 ggccacaggt ttcccgcatc ccacacccct gcacaagcta gggccctcta cacaagacac
      721 ctgctccggc tgaccgcaga caccagggct gcagcagtgc ccctcgaccc cgtgctgaat
      781 ccccagacaa atgctcctac acctctgggc ggagctgtcc tcagagctac at
//
