LOCUS       sequence-103455-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Destination vector for use 
            with pMuLE Entry vectors. Co-expresses EGFP under the control of a 
            PGK promoter. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE Lenti Dest eGFP
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             75..308
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(469..490)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gttcctctca ctctctgata ttcatttctt tgcaagttat aaatactgaa taataagatg
       61 acatgaacta ctactgctag agattttcca cactgactaa aagggtctga gggatctcta
      121 gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta
      181 ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc
      241 taaccagaga gacccagtac aagcaaaaag cagatcttgt cttcgttggg agtgagctag
      301 cccttccagt cccccctttt cttttaaaaa gtggctaaga tctacagctg ccttgtaagt
      361 cattggtctt aaaggtaccg ggccctcgag atgcatacgc gtcggctcta gagcgcggcc
      421 gctttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
      481 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag
      541 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      601 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat
      661 gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag
      721 cttgtg
//
