LOCUS       sequence-103460-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses red fluorescent protein, mKate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Davidsohn N, Beal J, Kiani S, Adler A, Yaman F, Li Y, Xie Z, Weiss R
  TITLE     Accurate Predictions of Genetic Circuit Behavior from Part 
            Characterization and Modular Composition.
  JOURNAL   ACS Synth Biol. 2014 Nov 17.
  PUBMED    25369267
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Nov 17."
COMMENT     Sequence Label: pExp_CAG:mKate
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    61..85
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(87..800)
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     regulatory      797..806
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggccacacca gccaccacct tctgataggc agcctgcacc tgaggagtga attcacgcgt
       61 accactttgt acaagaaagc tgggtatcaa ttaagtttgt gccccagttt gctagggagg
      121 tcgcagtatc tggccacagc cacctcgtgc tgctcgacgt aggtctcttt gtcggcctcc
      181 ttgattcttt ccagtcttct gtccacatag tagacgccgg gcatcttgag gttcttagcg
      241 ggtttcttgg atctgtatgt ggtcttcaag ttgcagatca ggtggccccc gcccacgagc
      301 ttcagggcca tgtcgcttct gccttccagg ccgccgtcag cggggtacag catctcggtg
      361 gaggcctccc agccgagtgt tttcttctgc atcacagggc cgttggatgg gaagttcacc
      421 cctctgatct tgacgttgta gatgaggcag ccgtcctgga ggctggtgtc ctgggtagcg
      481 gtcagcacgc ccccgtcttc gtatgtggtg actctctccc atgtgaagcc ctcagggaag
      541 gactgcttaa agaagtcggg gatgccctgg gtgtggttga tgaaggtttt gctgccgtac
      601 atgaagctgg tagccaggat gtcgaaggcg aaggggagag ggccgccctc gaccaccttg
      661 attctcatgg tctgggtgcc ctcgtagggc ttgccttcgc cctcggatgt gcacttgaag
      721 tggtggttgt tcacggtgcc ctccatgtac agcttcatgt gcatgttctc cttaatcagc
      781 tcttcgccct tagacaccat ggtggcgatc agcctgcttt t
//
