LOCUS       sequence-103461-         342 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK 
            RNA polymerase III promoter for shRNA expression. Compatible with 
            MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR 7SK L1-L4
FEATURES             Location/Qualifiers
     source          1..342
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    27..51
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgatgagca atgctttttt ataatgccaa ctttgtacaa aaaagcaggc tctatcgatc
       61 tagtgctgca gtatttagca tgccccaccc atctgcaagg cattctggat agtgtcaaaa
      121 cagccggaaa tcaagtccgt ttatctcaaa ctttagcatt ttgggaataa atgatatttg
      181 ctatgctggt taaattagat tttagttaaa tttcctgctg aagctctagt acgataagta
      241 acttgaccta agtgtaaagt tgagatttcc ttcaggttta tatagcttgt gcgccgcctg
      301 ggtacctcgg tcttcacaga tctagagctc ttttttgaat tc
//
