LOCUS       sequence-103463-         400 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human p53 (1-90) (72P) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Borcherds W, Theillet FX, Katzer A, Finzel A, Mishall KM, Powell AT,
            Wu H, Manieri W, Dieterich C, Selenko P, Loewer A, Daughdrill GW
  TITLE     Disorder and residual helicity alter p53-Mdm2 binding affinity and 
            signaling in cells.
  JOURNAL   Nat Chem Biol. 2014 Dec;10(12):1000-2. doi: 10.1038/nchembio.1668. 
            Epub 2014 Nov 2.
  PUBMED    25362358
REFERENCE   2  (bases 1 to 400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1668"; journalName: "Nat Chem Biol"; date: 
            "2014-12"; volume: "10"; issue: "12"; pages: "1000-2"
COMMENT     Sequence Label: pET28-p53 (1-90) (72P)
FEATURES             Location/Qualifiers
     source          1..400
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             373..390
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attttgttta ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgatggaag aacctcaatc agacccctca
      121 gttgaacccc ccttaagcca agaaaccttt tcagatttat ggaaattact cccagaaaat
      181 aatgtactct cccctctccc ctctcaagct atggacgact taatgctttc acccgatgat
      241 attgaacaat ggtttaccga agatcctgga cccgatgaag ccccacgtat gcctgaagcc
      301 gcccctcctg ttgcccccgc ccctgctgcc cctacccctg ctgcccccgc ccccgccccc
      361 tcttaactcg agcaccacca ccaccaccac tgagatccgg
//
