LOCUS       sequence-103469-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human p53 (1-73) (72R) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Vise PD, Baral B, Latos AJ, Daughdrill GW
  TITLE     NMR chemical shift and relaxation measurements provide evidence for 
            the coupled folding and binding of the p53 transactivation domain.
  JOURNAL   Nucleic Acids Res. 2005 Apr 11;33(7):2061-77. Print 2005.
  PUBMED    15824059
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2005-04-11"; volume: "33(7)"; pages: "2061-77. 
            Print 2005"
COMMENT     Sequence Label: pET28-p53 (1-73) (72R)
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..94
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             329..346
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(399..417)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      413..460
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(584..603)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     794..815
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tctgaatatt ttgtttactt taagaaggag atataccatg ggcagcagcc atcatcatca
       61 tcatcacagc agcggcctgg tgccgcgcgg cagccatatg atggaggagc cgcagtcaga
      121 tcctagcgtc gagccccctc tgagtcagga aacattttca gacctatgga aactacttcc
      181 tgaaaacaac gttctgtccc ccttgccgtc ccaagcaatg gatgatttga tgctgtcccc
      241 ggacgatatt gaacaatggt tcactgaaga cccaggtcca gatgaagctc ccagaatgcc
      301 agaggctgct ccccgcgtgt gactcgagca ccaccaccac caccactgag atccggctgc
      361 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      421 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      481 cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
      541 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
      601 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      661 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
      721 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
      781 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
      841 tattc
//
