LOCUS       sequence-103470-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human p53 (1-73) (72R) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Vise PD, Baral B, Latos AJ, Daughdrill GW
  TITLE     NMR chemical shift and relaxation measurements provide evidence for 
            the coupled folding and binding of the p53 transactivation domain.
  JOURNAL   Nucleic Acids Res. 2005 Apr 11;33(7):2061-77. Print 2005.
  PUBMED    15824059
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2005-04-11"; volume: "33(7)"; pages: "2061-77. 
            Print 2005"
COMMENT     Sequence Label: pET28-p53 (1-73) (72R)
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(284..301)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(311..328)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             347..369
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    384..408
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(408..427)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(409..427)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(511..530)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttccttcggg cttgtagcag ccggatctca gtggtggtgg tggtggtgct cgagtcacac
       61 gcggggtagc agcctctggc attctgggag cttcatctgg acctgggtct tcagtgaacc
      121 attgttcaat atcgtccggg gacagcatca aatcatccat tgcttgggac ggcaaggggg
      181 acagaacgtt gttttcagga agtagtttcc ataggtctga aaatgtttcc tgactcagag
      241 ggggctcgac gctaggatct gactgcggct cctccatcat atggctgccg cgcggcacca
      301 ggccgctgct gtgatgatga tgatgatggc tgctgcccat ggtatatctc cttcttaaag
      361 ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt
      421 cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc gtggccggca
      481 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
      541 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
      601 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
      661 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
      721 agcgtcgaga tcccggacac catcgaatgg cgc
//
