LOCUS       sequence-103471-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human p53 (1-73) (72R) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Vise PD, Baral B, Latos AJ, Daughdrill GW
  TITLE     NMR chemical shift and relaxation measurements provide evidence for 
            the coupled folding and binding of the p53 transactivation domain.
  JOURNAL   Nucleic Acids Res. 2005 Apr 11;33(7):2061-77. Print 2005.
  PUBMED    15824059
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2005-04-11"; volume: "33(7)"; pages: "2061-77. 
            Print 2005"
COMMENT     Sequence Label: pET28-p53 (1-73) (72R)
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(283..300)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(310..327)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             346..368
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    383..407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(407..426)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(408..426)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(510..529)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttccttcggg cttgtagcag ccggatctca gtggtggtgg tggtggtgct cgagtcacac
       61 gcggggagca gcctctggca ttctgggagc ttcatctgga cctgggtctt cagtgaacca
      121 ttgttcaata tcgtccgggg acagcatcaa atcatccatt gcttgggacg gcaaggggga
      181 cagaacgttg ttttcaggaa gtagtttcca taggtctgaa aatgtttcct gactcagagg
      241 gggctcgacg ctaggatctg actgcggctc ctccatcata tggctgccgc gcggcaccag
      301 gccgctgctg tgatgatgat gatgatggct gctgcccatg gtatatctcc ttcttaaagt
      361 taaacaaaat tatttctaga ggggaattgt tatccgctca caattcccct atagtgagtc
      421 gtattaattt cgcgggatcg agatctcgat cctctacgcc ggacgcatcg tggccggcat
      481 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
      541 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
      601 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
      661 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
      721 gcgtcgagat cccggacacc atcgaatggc gc
//
