LOCUS       sequence-103472-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human ubc13, E2 for synthesis k63 linked ubiquitin chain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Wiener R, Zhang X, Wang T, Wolberger C
  TITLE     The mechanism of OTUB1-mediated inhibition of ubiquitination.
  JOURNAL   Nature. 2012 Feb 22;483(7391):618-22. doi: 10.1038/nature10911.
  PUBMED    22367539
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-02-22- 22"; volume: "483"; issue: "7391"; 
            pages: "618-22"
COMMENT     Sequence Label: hUbc13
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    67..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             106..128
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             142..159
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gatgcgtccg gcgtagagga tcgagatcga tctcgatccc gcgaaattaa tacgactcac
       61 tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
      121 gaaggagata tacatatggg ccatcatcat catcatcatc cgtcaggagt caagactgag
      181 aacaacgatc atattaattt gaaggtggcg gggcaggatg gttctgtggt gcagtttaag
      241 attaagaggc atacaccact tagtaaacta atgaaagcct attgtgaacg acagggattg
      301 tcaatgaggc agatcagatt ccgatttgac gggcaaccaa tcaatgaaac agacacacct
      361 gcacagttgg aaatggagga tgaagataca attgatgtgt tccaacagca gacgggagga
      421 atggccgggc tgccccgcag gatcatcaag gaaacccagc gtttgctggc agaaccagtt
      481 cctggcatca aagccgaacc agatgagagc aacgcccgtt attttcatgt ggtcattgct
      541 ggccctcagg attccccctt tgagggaggg acttttaaac ttgaactatt ccttccagaa
      601 gaatacccaa tggcagcccc taaagtacgt ttcatgacca aaatttatca tcctaatgta
      661 gacaagttgg gaagaatatg tttagatatt ttgaaagata agtggtcccc agcactgcag
      721 atccgcacag ttctgctatc gatccaggcc ttgttaagtg ctcccaatcc agatgatcca
      781 ttagcaaatg atgtagcgga gca
//
